Application of soybean GmSPA3a/3b protein and related biological materials thereof in regulation and control of plant flowering and plant height
A biological material and plant technology, applied in the biological field, can solve the problems of unclear molecular mechanism of soybean, low lodging and seed setting rate, etc.
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Embodiment 1
[0110] Embodiment 1, the acquisition of GmSPA3a / 3b gene deletion mutant plants
[0111] 1. Construction of CRISPR-Cas9 recombinant plasmids GmSPA3a-gRNA1 and GmSPA3a-gRNA2
[0112] The GmSPAs genes in the soybean genome include a pair of homologous genes GmSPA3a and GmSPA3b (hereinafter referred to as GmSPA3a / 3b). The amino acid sequences of the GmSPA3a and GmSPA3b proteins in the soybean William 82 genome are respectively shown in Sequence 1 and Sequence 2 in the sequence listing, and the corresponding CDS coding sequences of GmSPA3a and GmSPA3b are the nucleotides shown in Sequence 3 and Sequence 4 in the sequence listing respectively .
[0113] 1.1. Obtaining U6-gRNA
[0114] According to the genome sequence of soybean protein GmSPA3a / 3b, a single guide RNA (single guide RNA) target sequence was designed, and the designed sgRNA targeting GmSPA3a was named sgRNA1. The designed gRNA target sequence was compared with the soybean genome sequence, to exclude non-specific targ...
Embodiment 2
[0172] Example 2. Application of GmSPA3a / 3b gene in regulating soybean flowering and plant height
[0173] 1. Observation of flowering time and plant height phenotype in the field
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