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Application of soybean GmSPA3a/3b protein and related biological materials thereof in regulation and control of plant flowering and plant height

A biological material and plant technology, applied in the biological field, can solve the problems of unclear molecular mechanism of soybean, low lodging and seed setting rate, etc.

Active Publication Date: 2021-11-12
LONGPING BIOTECHNOLOGY (HAINAN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-latitude areas usually require soybean varieties with short stems and short growth periods. As an excellent variety in low-latitude areas, TL1 will suffer from severe lodging and low seed-setting rate in high-latitude areas.
[0004] In 2010, scientists completed the sequencing of the soybean genome, which provided conditions for the study of gene functions. It is more convenient to clone good genes in soybeans, but the molecular mechanism of soybean plant type is still unclear

Method used

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  • Application of soybean GmSPA3a/3b protein and related biological materials thereof in regulation and control of plant flowering and plant height
  • Application of soybean GmSPA3a/3b protein and related biological materials thereof in regulation and control of plant flowering and plant height
  • Application of soybean GmSPA3a/3b protein and related biological materials thereof in regulation and control of plant flowering and plant height

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Embodiment 1, the acquisition of GmSPA3a / 3b gene deletion mutant plants

[0111] 1. Construction of CRISPR-Cas9 recombinant plasmids GmSPA3a-gRNA1 and GmSPA3a-gRNA2

[0112] The GmSPAs genes in the soybean genome include a pair of homologous genes GmSPA3a and GmSPA3b (hereinafter referred to as GmSPA3a / 3b). The amino acid sequences of the GmSPA3a and GmSPA3b proteins in the soybean William 82 genome are respectively shown in Sequence 1 and Sequence 2 in the sequence listing, and the corresponding CDS coding sequences of GmSPA3a and GmSPA3b are the nucleotides shown in Sequence 3 and Sequence 4 in the sequence listing respectively .

[0113] 1.1. Obtaining U6-gRNA

[0114] According to the genome sequence of soybean protein GmSPA3a / 3b, a single guide RNA (single guide RNA) target sequence was designed, and the designed sgRNA targeting GmSPA3a was named sgRNA1. The designed gRNA target sequence was compared with the soybean genome sequence, to exclude non-specific targ...

Embodiment 2

[0172] Example 2. Application of GmSPA3a / 3b gene in regulating soybean flowering and plant height

[0173] 1. Observation of flowering time and plant height phenotype in the field

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Abstract

The invention discloses application of soybean GmSPA3a / 3b protein and related biological materials thereof to regulation and control of plant flowering and plant height. The technical scheme protected by the invention is the application of the protein related biological material in regulating or shortening the flowering phase of the plant, regulating or reducing the plant height of the plant and breeding and improving the plant. The amino acid sequence of the protein can be shown as a sequence 1 or a sequence 2 in a sequence table. Experiments prove that the transgenic soybean Gmspa3a, Gmspa3b and Gmspa3ab mutants with the GmSPA3a / 3b protein activity lost show the phenotype of advanced flowering phase and dwarf plant height. By knocking out the soybean Gmspa3a, Gmspa3b or GmSPA3a / 3b gene, the photoperiod sensitivity of the soybean can be reduced, and a plurality of strains with different flowering times and plant heights can be cultivated to adapt to planting in different areas, so that the sowing area of the excellent soybean is effectively expanded, and yield increase and harvest are realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of soybean GmSPA3a / 3b protein and related biological materials in regulating plant flowering and the number of main stem nodes. Background technique [0002] Soybean (Glycine max) is an important source of edible vegetable oil and vegetable protein in the world. Light is one of the most important environmental factors affecting soybean growth and development. Soybean is a typical short-day plant, and most soybean varieties have narrow adaptability to photoperiods at different latitudes. [0003] Crop type refers to the overall manifestation of the plant's morphological layout, ecological characteristics and physiological characteristics. An ideal plant type can increase the leaf area coefficient of the crop, increase the photosynthetic efficiency of the group, improve the relationship between the crop's demand for fertilizers, and thus increase the yield...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N15/113A01H5/02A01H5/00A01H6/54
CPCC07K14/415C12N15/113C12N15/8218C12N15/827C12N15/8261C12N2310/20Y02A40/146
Inventor 赵涛秦超李宏宇刘斌刘军
Owner LONGPING BIOTECHNOLOGY (HAINAN) CO LTD
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