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Application of oxfendazole as anti-ovarian cancer drug

A technology for ovarian cancer and ovarian cancer cells, applied in the field of medicine, to achieve the effect of good anti-ovarian cancer and inhibition of proliferation

Pending Publication Date: 2021-11-16
DONGGUAN PEOPLES HOSPITAL
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the anti-ovarian cancer of oxfendazole

Method used

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  • Application of oxfendazole as anti-ovarian cancer drug
  • Application of oxfendazole as anti-ovarian cancer drug
  • Application of oxfendazole as anti-ovarian cancer drug

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0024] Experimental Example 1 Oxfendazole inhibits the viability of ovarian cancer cells

[0025] Ovarian cancer cells (A2780, OVCAR3, SKOV3, and KGN) in the logarithmic growth phase purchased from Beina Biological Company were inoculated in a 96-well plate with 5000 cells per well and 200 μL of RPMI-1640 medium. Set up drug treatment group, control group and blank group respectively. Among them, the drug treatment group is a group containing different concentrations of oxfendazole (10 μM, 25 μM, 50 μM, 100 μM), the control group only contains cells without drugs, and the blank group contains no cells and only medium. In the drug treatment group, the cells were treated with drugs for 48 hours, and 4 hours before the end of the treatment, 20 μL of CCK8 solution was added to each well and cultured for 4 hours. Then read the absorbance value at a wavelength of 450nm with a full-wavelength multifunctional microplate reader, and calculate the cell viability according to the follow...

experiment example 2

[0028] Experimental Example 2 Inhibitory Effect of Oxfendazole on the Proliferative Ability of Ovarian Cancer Cells

[0029] Ovarian cancer cells (A2780) in the logarithmic growth phase were inoculated in a six-well plate with 500 cells per well and 200 μL of medium. After the cells were completely attached to the wall, the cells were treated with different concentrations of oxfendazole (7.5 μM and 10 μM) for 7 days. The medium was changed every 3 days during the process. After the treatment, the cells were discarded of the medium, washed twice with PBS, fixed with methanol in an ice bath for 5 min and stained with crystal violet.

[0030] like figure 2 The results showed that oxfendazole can inhibit the proliferation ability of ovarian cancer cells.

experiment example 3

[0031] Experimental Example 3 Induction of Ovary Cancer Cell Apoptosis by Oxfendazole

[0032] Ovarian cancer cells (A2780) in the logarithmic growth phase were treated with different concentrations of oxfendazole (5 μM and 10 μM) for 48 hours, digested with EDTA-free trypsin for 2 minutes to single cells, centrifuged at 2000 rpm for 5 minutes after digestion, and washed with PBS for 2 minutes Second-rate. Then add the apoptosis detection kit working solution (500 μL binding buffer, 5 μL Annexin V-FITC dye and 5 μL PI dye) purchased from Jiangsu KGI Biotechnology Co., Ltd. to the cells, mix well and place in the dark for 15 minutes. . The ratio of apoptosis was detected by BD FACSCanto II flow cytometer. Among them, Annexin V-FITC single-positive cells and Annexin V-FITC / PI double-positive cells were counted as apoptotic cells.

[0033] like image 3 The results showed that oxfendazole can induce apoptosis in ovarian cancer cells.

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to application of oxfendazole as an ovarian cancer resisting drug; in order to research and develop a new ovarian cancer resisting drug, research finds that oxfendazole can obviously inhibit the activity of ovarian cancer cells from different sources; in addition, the proliferation of ovarian cancer cells can be inhibited, and apoptosis of the ovarian cancer cells can be induced; it is proved that oxfendazole has a very good ovarian cancer resisting effect and is expected to be developed into a brand-new efficient medicine for treating ovarian cancer, so that the new application of oxfendazole is explored, and a new medicine and a new way are provided for treating ovarian cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of oxfendazole as an anti-ovarian cancer drug. Background technique [0002] Ovarian cancer is a common malignant tumor of the female reproductive system. In recent years, the incidence and mortality of ovarian cancer have been on the rise. Worldwide, the incidence of ovarian cancer ranks third among malignant tumors of the female reproductive system, and its mortality rate ranks second. At present, the treatment of ovarian cancer is mainly a combination of surgery and chemotherapy. As far as chemotherapy is concerned, with the continuous development of modern medical research, chemotherapy drugs for the treatment of ovarian cancer have also been continuously developed. The chemotherapy drugs currently used in the clinical treatment of ovarian cancer mainly include paclitaxel, carboplatin, and doxorubicin. The application of chemotherapy drugs has s...

Claims

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Application Information

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IPC IPC(8): A61K31/4184A61K45/06A61P35/00
CPCA61K31/4184A61K45/06A61P35/00
Inventor 温创宇李仲均吴书仪汤雯婷吴寰雨欧宜静李琳陈锐聪黄素然
Owner DONGGUAN PEOPLES HOSPITAL
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