Whole genome typing method based on DNA methylation site genotypes

Abstract

The invention discloses a whole genome typing method based on DNA methylation site genotypes. The whole genome typing method comprises the following steps: step 1, collecting a sample; step 2, establishing a DNA library; step 3, sequencing the library; step 4, identifying a site; and step 5, genotyping. In the step 2, MBD treatment of the combined methylation sensitive restriction endonuclease refers to that the endonuclease of a non-to-be-detected area and the methylation sensitive restriction endonuclease are used for digesting DNA fragments at the same time, then fragments containing methylation areas are captured and reserved through an MBD column, and in the step 1, at different time periods such as daytime and night in a group, compared with an existing genotyping method, the method has the advantages that the influence of environments in different time periods on the DNA methylation state can be judged; according to the invention, the change of the DNA methylation state in different growth states can be judged; and according to the method, two different methods for treating DNA are adopted, so that the genotyping accuracy is improved.

Description

Technical field [0001] The present invention relates to the field of DNA methylation techniques, in particular a whole genome component type method based on a DNA methylation site genotype. Background technique [0002] DNA methylation is a form of chemical modification of DNA, which can change genetic performance without changing the DNA sequence, exhibiting cytosine of genomic CPG di-nuclei under the action of DNA methylated transferase The 5 'carbon position covalent bond binds a methyl group, and the existing genometric raw sulfite sequencing method is unable to determine the effect of the environmental effect on the DNA methylation state when the genotype is classified. The sequencing method cannot determine the change in DNA methylation site genotype under different growth states; existing sequencing methods only use heavy bisulfite sequencing, not accurate enough. Inventive content [0003] It is an object of the present invention to provide a whole genome component type ...

AI Technical Summary

Problems solved by technology

[0002] DNA methylation is a form of chemical modification of DNA, which can change genetic expression without changing the DNA sequence. The 5' carbon position is covalently bonded to a methyl group. The existing genome-wide bisulfite sequencing method cannot judge the impact of environmental effects on the DNA methylation status when classifying genotypes; the existing Sequencing methods cannot determine the genotype changes of DNA methylation sites under different growth states; existing sequencing methods only use bisulfite sequencing, which is not accurate enough

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