Sample injection chip and method based on electrophoresis technology

Abstract

The invention provides a sample injection chip and a sample injection method based on an electrophoresis technology, the sample injection chip comprises a substrate and an electrode, and the substrate is made of an insulating material; the sample injection chip further comprises a temporary storage module which is arranged on the substrate and is used for temporarily storing samples; and the electrode is annular and is arranged around the temporary storage module. The sample injection chip has the advantages of few required samples and the like.

Description

technical field [0001] The invention relates to pollution monitoring, in particular to a sample injection chip and method based on electrophoresis technology. Background technique [0002] Capillary electrophoresis is a widely used and efficient detection technique that can be applied to the separation and detection of a variety of inorganic and organic substances. The capillary electrophoresis sampling process is one of the important steps that affect the reproducibility and sensitivity of the analysis, and electrosampling is a widely used method. In the process of electrosampling, it is necessary to penetrate the metal electrode deep into the liquid to be tested, and after applying voltage, the charged substances can migrate into the separation pipe to realize the process of sampling. Limited by the existing electrosampling method, the volume of the substance to be detected needs to be greater than 10 microliters, while the actual injection volume of capillary electrophor...

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Problems solved by technology

[0003] 1. Waste of some expensive samples;

[0004] 2. Unable to analyze trace samples;

[0005] 3. It is difficult to miniaturize the capillary electrophoresis instrument;

[0006] 4. Unable to connect with the upstream micro reaction system (microfluidic)

[0008] 1. SCIEX places an electrode at a distance of about 2 mm from the sampling end of the capillary. When the sample is injected, the electrode and the capillary penetrate into the liquid to be tested together to realize electrical sampling, but this method is not suitable for the analysis of trace liquids;

[0009] 2. Thermo Fisher uses the method of fixing the tip of the capillary to the metal sleeve to realize that the electrode and the capillary enter the sample together, but due to the large volume of the sleeve, a small amount of the liquid to be measured will be adsorbed to the sleeve and affect subsequent analysis;

[0010] 3. Put the droplets on the metal plate for capillary electrosampling, but because the metal plate has a strong inhibitory effect on many biological reactions, it limits the connection with different upstream reactions

[0011] The method based on the microfluidic chip can realize the capillary electrophoresis analysis of the micro-reaction system, but most of these chips need a high-precision syringe pump as a power source to assist the injection of micro-sample. This method not only requires additional equipment but also has low repeatability. it is good

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