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A molecular marker g2997 for rapid identification of low temperature tolerant varieties of Penaeus japonicus and its application

A technology of G2997-R and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of lack of molecular markers, and achieve accurate and reliable methods, simple operation, and improved nutrition. The effect of success rate and breeding cycle

Active Publication Date: 2022-04-22
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the development of molecular markers for low temperature tolerance of Penaeus japonicus, and there is a lack of markers that can be applied to molecular marker-assisted breeding in the industry

Method used

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  • A molecular marker g2997 for rapid identification of low temperature tolerant varieties of Penaeus japonicus and its application
  • A molecular marker g2997 for rapid identification of low temperature tolerant varieties of Penaeus japonicus and its application
  • A molecular marker g2997 for rapid identification of low temperature tolerant varieties of Penaeus japonicus and its application

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Embodiment 1

[0029] 1. Screening of candidate molecular markers related to low temperature resistance

[0030] 1. Sequencing data filtering and comparison

[0031] Genomic DNA of Penaeus japonicus was extracted from 20 individuals in each of the low temperature tolerance group and the low temperature intolerant group. Genomic DNA of Penaeus japonicus was extracted by using a centrifugal adsorption column that can specifically bind DNA and a unique buffer system. First, put about 30 mg of tissue samples into a 1.5 mL sterile enzyme centrifuge tube, add 200 μL buffer GA and 20 μL Proteinase K solution, vortex to mix, and incubate at 56°C until the tissue is completely lysed. Then add 200 μL buffer GB, mix well by inverting, place at 70°C for 10 min, the solution becomes clear, and briefly centrifuge to remove water droplets on the inner wall. Then add 200 μL of absolute ethanol, invert and mix thoroughly. At this time, flocculent precipitates may appear, centrifuge briefly, and then add th...

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Abstract

The invention provides a molecular marker G2997 for quickly identifying low-temperature-tolerant species of Penaeus japonicus and its application. The nucleotide sequence of the molecular marker G2997 is shown in SEQ ID No.1, wherein the 501st base is A. The molecular marker G2997 is a SNP marker, and its low temperature tolerance genotype is GA genotype. The molecular marker G2997 and its detection primers can not be limited by the developmental stages of Penaeus japonicus, significantly speed up the breeding process of Penaeus japonicus and quickly breed new varieties of Penaeus japonicus with excellent low temperature tolerance, and use the molecular marker G2997 is accurate, reliable and easy to operate in detecting the low temperature resistance of Penaeus japonicus, which is beneficial to the healthy breeding and development of Penaeus japonicus, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of molecular marker assisted breeding of aquatic animals, and in particular relates to a molecular marker G2997 for quickly identifying low temperature tolerant species of Penaeus japonicus and its application. Background technique [0002] Japanese prawns ( Marsupenaeus japonicus ), formerly known as Penaeus japonicus, belongs to Decapod, Penaeidae, and Penaeus genus. It is distributed in the tropical Indo-West Pacific, the east coast of Africa, Malaysia, Japan, North Korea, and the southeast coast of China. It is one of the main shrimp farming species in China. Penaeus japonicus grows rapidly, is resistant to dry dew, bright in color and has high economic value. It is popular in the aquatic product market and farmers. It is cultivated in various provinces and cities from the south to the north of my country, and has formed a certain scale. farming is of great importance. Low temperature tolerance is an important st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 贾少婷任宪云邵慧鑫孟宪亮王琼徐垚刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI