Quadruple quantitative fluorescent probe primer combination, kit and identification method for identifying African swine fever wild virus and vaccine strain

A technology of African swine fever virus and fluorescent probe, applied in the field of quadruple quantitative fluorescent probe primer combination, can solve the problems of complex immune evasion mechanism, lack of neutralizing antibody, and no treatment measures
CN113755565APending Publication Date: 2021-12-07FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD +3
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUJIAN AONONG BIOLOGICAL TECH GRP CO LTD
Publication Date
2021-12-07

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Abstract

The invention discloses a quadruple quantitative fluorescent probe primer combination, a kit and an identification method for identifying an African swine fever wild virus and a vaccine strain, and belongs to the technical field of molecular biology. The quadruple quantitative fluorescent probe primer combination for identifying the African swine fever wild virus and the vaccine strain comprises: (a) a first primer probe combination as shown in SEQ ID NO:1-SEQ ID NO:3; (b) a second primer probe combination as shown in SEQ ID NO:4 to SEQ ID NO:6; (c) a third primer probe combination as shown in SEQ ID NO:7-SEQ ID NO:9; and (d) a fourth primer probe combination as shown in SEQ ID NO:10 to SEQ ID NO:12. The kit and the identification method can simultaneously identify four genes of the African swine fever wild strain and the vaccine strain, primers of different genes do not interfere with each other, and the kit and the identification method have the characteristics of high sensitivity, strong specificity, good repeatability and good stability.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology, and in particular relates to a quadruple quantitative fluorescent probe primer combination, kit and identification method for identifying African swine fever wild virus and vaccine strains. Background technique

[0002] African swine fever (ASF) is an acute, febrile, highly contagious disease of pigs caused by African swine fever virus (ASFV). Domestic pigs and wild boars are generally susceptible, and soft ticks are the storage host and medium of ASFV. The incubation period of ASFV is 5-15 days. Once an outbreak occurs, the course of disease is short and the case fatality rate is high, which can reach 100%. The on-site symptoms are complex and difficult to distinguish from other diseases, mostly manifested as high fever, skin congestion, miscarriage, edema, and organ hemorrhage. However, there is no effective treatment after the onset of African swine fever, and the virus has a compl...

Claims

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