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Application of urine immunoglobulin heavy chain variable region 3-7 and polypeptide fragment thereof in burns

An immunoglobulin and polypeptide fragment technology, applied in the field of urinary immunoglobulin heavy chain variable regions 3-7 and their polypeptide fragments, can solve the problems of poor skin integrity, aggravate the pain of patients, etc., and achieve the effect of convenient storage

Pending Publication Date: 2021-12-17
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the integrity of the skin of patients with severe burns is poor. As an invasive skin test, there are many difficulties in the clinical application of blood tests for such patients. Repeated blood testing will also aggravate the suffering of patients.

Method used

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  • Application of urine immunoglobulin heavy chain variable region 3-7 and polypeptide fragment thereof in burns
  • Application of urine immunoglobulin heavy chain variable region 3-7 and polypeptide fragment thereof in burns
  • Application of urine immunoglobulin heavy chain variable region 3-7 and polypeptide fragment thereof in burns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Urine Specimen Collection and Processing

[0022] The burn patients were selected as the burn group, and the healthy subjects who underwent physical examination during the same period were selected as the normal control group. Collect 30ml of fresh morning urine samples from the research subjects in each group after admission, and collect the urine in the catheter in the morning for those who cannot urinate normally, and place them in a dry and clean container. After the collected urine samples were centrifuged at 4000r / min for 5min, the supernatant was aspirated, divided into 2ml tubes, and stored in a -80°C refrigerator.

Embodiment 2

[0023] Example 2 Mass Spectrometry and Screening of Urine Peptides

[0024] Extract protein from urine samples and determine the concentration of the extracted protein. Mass spectrometric analysis of urine samples was performed by an OrbitrapFusion type mass spectrometer. Quantitative calculation was performed on the data obtained in the mass spectrometer of the experimental group and the normal control group. The difference between groups was analyzed by t-test, and the difference in protein expression was more than 1.5 times and the statistical test P<0.05 was used as the reference standard to screen for differentially expressed proteins.

Embodiment 3

[0025] Example 3 Identification and Analysis of Differential Peptides

[0026] The database used this time is the Uniprot_Homo database, and the original mass spectrum files generated are processed by MaxQuant software, and the search parameter settings are shown in Table 1.

[0027]

[0028] Immunoglobulin heavy chain variable domains 3–7 are underexpressed in the urine of burn patients compared with healthy individuals, as shown in figure 1 As shown, the main biological processes involved in it are as follows figure 2 As shown, there is a significant difference in the expression of immunoglobulin heavy chain variable region 3-7 in the urine of the normal control group and the burn group.

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Abstract

The invention provides application of a urine immunoglobulin heavy chain variable region 3-7 (Immunoglobulin heavy variable 3-7) and a polypeptide fragment thereof, and particularly relates to the application of the urine immunoglobulin heavy variable 3-7 and the polypeptide fragment thereof in preparing preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like. Burns are common important wounds in daily life, about 5000-100000 people in every one million people are burnt every year, according to statistics of World Health Organization, more than 300,000 patients die from burns globally every year, and the treatment survival rate of severe burn patients is still at a lower level. Researches prove that compared with a healthy person (a normal control group), the urine immunoglobulin heavy variable 3-7 and the polypeptide fragment thereof have down-regulated expression in burn patients. The urine immunoglobulin heavy variable 3-7 and the polypeptide fragment thereof can be used for auxiliary diagnosis and illness monitoring of burn patients. According to the application, the advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of a urine sample are exerted, and the urine immunoglobulin heavy variable 3-7 and the polypeptide fragment thereof are detected by using the urine sample.

Description

technical field [0001] The present invention relates to new uses of urinary immunoglobulin heavy chain variable regions 3-7 and polypeptide fragments thereof, in particular to the use of urine immunoglobulin heavy chain variable regions 3-7 and polypeptide fragments in burn diagnosis, differential diagnosis, The application of burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation and mechanism research. Background technique [0002] Burn refers to the damage of human skin, tissues and even deep organs caused by chemical substances such as flame, high temperature gas, hot solid or liquid, radiation, electric energy, strong acid and strong alkali, and is a systemic comprehensive disease. Massive necrosis of wound tissue, infection, shock, and coagulation dysfunction after burns can cause a series of pathophysiological changes in the body. Different degrees of burns have different effects on the human body. Severe burns can cause damage...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543C12Q1/6883
CPCG01N33/6893G01N33/54306G01N33/6848G01N33/6854C12Q1/6883C12Q2600/158G01N2800/52G01N2800/40
Inventor 张曼王佶图
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY