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SSR molecular marker of major QTL as well as screening method and application of SSR molecular marker

A technology of molecular markers and sites, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problems of low versatility and different identification efficiency

Active Publication Date: 2021-12-21
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of SSR molecular marker and its application for identifying the seedless main effect QTL locus of grape fruit, which can effectively solve the problem of different identification efficiency of existing seedless molecular markers in different types of seedless grapes and different populations. The problem of low versatility

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  • SSR molecular marker of major QTL as well as screening method and application of SSR molecular marker
  • SSR molecular marker of major QTL as well as screening method and application of SSR molecular marker
  • SSR molecular marker of major QTL as well as screening method and application of SSR molecular marker

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Embodiment Construction

[0027] In order to make the objects and advantages of the present invention clearer, the present invention will be specifically described below in conjunction with examples. It should be understood that the following words are only used to describe one or several specific implementation modes of the present invention, and do not strictly limit the protection scope of the specific claims of the present invention.

[0028] Development of the molecular marker P1_VvMETK5

[0029] According to the sequences of non-nuclear molecular markers that have been counted, a total of 1261 genes were counted within 1 Kb of the non-nuclear molecular markers. Using GA-Fujiminori (GA 3 treatment) and CK-Fujiminori (Shimizu treatment) seed / nuclear region transcriptome data, using the K-means clustering algorithm (K-means) to cluster CK-Fujiminori and GA-Fujiminori in seed development differential genes analyze.

[0030] 41 genes with a similar expression trend to the non-nuclear gene VvAGL11 w...

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Abstract

The invention relates to the field of grape molecular breeding, in particular to an SSR molecular marker of a major QTL as well as a screening method and application of the SSR molecular marker. According to the technical scheme, a novel SSR molecular marker P1VvMETK5 is screened by utilizing a specific screening method and is applied to identification of seedless of grape fruits. According to the technical scheme, the contribution rate of the locus for identifying seedless major QTL of the grape fruits is 78.95%, a linked SSR new molecular marker P1VvMETK5 is screened based on the locus, only aa / ab and AA / AB genotypes are amplified from nucleated and seedless varieties by the marker, the explanation rate of the seedless character ab heterozygous genotype in a natural population is 57.70%, and the explanation rate of the nucleated character BB genotype is 78.95%.

Description

technical field [0001] The invention relates to the technical field of grape molecular breeding, in particular to an SSR molecular marker of a major QTL site and a screening method and application thereof. Background technique [0002] Grapes are perennial deciduous woody vines. With the continuous renewal of grape varieties, seedless grapes occupy an important position in the fresh and dried grape market because of their advantages of high yield, high quality, and convenient consumption, and are also an important development direction of grape breeding. The current seedless grape breeding methods include: conventional cross breeding, bud mutation selection, polyploidy breeding, embryo rescue breeding and molecular marker assisted selection breeding. Because molecular marker-assisted selection is not affected by developmental stage, external environment and tissue specificity, it can be efficiently detected at the seedling stage, overcome the shortcoming of long hybrid bree...

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 王晨叶东东宣旭娴吴伟民王西成房经贵肖迎珂
Owner NANJING AGRICULTURAL UNIVERSITY