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Application of radix stemonae in preparation of product for inhibiting proliferation of intestinal flora

A technology of intestinal flora and 100 parts, applied in the field of microorganisms, to achieve the effect of efficient preparation

Inactive Publication Date: 2021-12-28
MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no relevant report on its regulation effect on intestinal microbiota

Method used

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  • Application of radix stemonae in preparation of product for inhibiting proliferation of intestinal flora
  • Application of radix stemonae in preparation of product for inhibiting proliferation of intestinal flora
  • Application of radix stemonae in preparation of product for inhibiting proliferation of intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Carry out in vivo simulation experiments in vitro, take human feces as intestinal flora samples, and observe the effect of Baibu intervention on intestinal flora. The specific operation process is as follows:

[0037] 1. Fecal sample processing

[0038] (1) PBS buffer preparation

[0039] Taking 1000ml as an example, weigh the following medicines with an electronic analytical balance and pour them into a 1000ml beaker, including: KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O2.90g, NaCl8.00g, KCl0.20g.

[0040] Use a graduated cylinder to measure 800ml of ultra-pure water and pour it into a beaker, put it into the stirring rotor, and put it on a magnetic heating stirrer to stir until it is completely dissolved, then use a calibrated pH meter to measure the pH of the above solution, use 0.1M HCl or NaOH adjusted pH=7.4±0.05.

[0041] Drain the above-mentioned completely dissolved and adjusted pH solution into a 1000ml volumetric flask with a glass rod, rinse the beaker with a...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses application of radix stemonae in preparation of a product for inhibiting proliferation of intestinal flora. According to the application, by simulating an intestinal environment in vitro and taking intestinal flora collected from faeces of a healthy human body as a sample, radix stemonae is applied to proliferation and fermentation of the intestinal flora, so that the radix stemonae is found to be capable of remarkably inhibiting proliferation of the intestinal flora; the radix stemonae has an extremely remarkable inhibition effect on intestinal flora such as Lachnospiraceae bacterium, Lachnoclostridium, Dorea, allisonella and desulphovibrio and in addition, the invention further provides application of the radix stemonae in preparation of medicines for treating or improving intestinal inflammatory diseases caused by intestinal flora proliferation. Scientific basis is provided for clinical application, and the application field of radix stemonae is expanded.

Description

technical field [0001] The present invention relates to the technical field of microbes, in particular to the application of cymbidium in the preparation of products for inhibiting the proliferation of intestinal flora. Background technique [0002] Baibu has the effects of moistening the lungs, lowering qi, relieving cough, killing insects and killing lice. Commonly used for chronic cough, pulmonary tuberculosis cough, sudden cough; externally used for head lice, body lice, pinworms, pruritus vulvae. Moisturizing the lungs and relieving cough, it is used for cough due to yin deficiency. However, there is no relevant report on its regulation effect on intestinal microbiota. [0003] There are a large number of microorganisms in the human intestinal tract. These microorganisms rely on the human intestinal tract to live and help the human body to complete various physiological and biochemical functions. Gut microbes not only serve as an important bridge between diet and hos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/904A61P1/00A61P29/00A61P35/00A61P1/10A61P31/04A23L33/105
CPCA61K36/904A61P1/00A61P29/00A61P35/00A61P1/10A61P31/04A23L33/105A23V2002/00A23V2200/32A23V2200/308A23V2250/21
Inventor 刘庆军沈鹤霄李国龙张帆彭文聪熊云刘慧敏吕永玲
Owner MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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