Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of sequencing primer, detection method and kit for dengue virus typing

A dengue virus and kit technology, applied in the field of biomedicine, can solve problems such as the inability to determine the virus type

Active Publication Date: 2022-05-13
CHINESE ACAD OF INSPECTION & QUARANTINE
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the dengue virus is monitored by the reaction pool method, false negatives still exist, and the virus type cannot be determined

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of sequencing primer, detection method and kit for dengue virus typing
  • A kind of sequencing primer, detection method and kit for dengue virus typing
  • A kind of sequencing primer, detection method and kit for dengue virus typing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Embodiment 1, detection method of the present invention

[0110] The operation process of the multiplex PCR_MS mass spectrometry scanning method of the present invention is as follows figure 1 ,details as follows:

[0111] (1) Establishment of dengue virus gene database: 4,502 complete genome sequences of dengue virus types I-IV were obtained from the NCBI website, including: 1,874 for type I, 1,478 for type II, 944 for type III and 206 for type IV. See Table 1 for details.

[0112] Table 1. Sequence source of primers designed for dengue virus DENV in PCR-MS

[0113] Table 1. Reference strains used in Multiplex PCR-MS design

[0114]

[0115]

[0116] (2) Establishment of SNP typing of dengue virus: After clustering analysis with Sequencher5.3 software, a type-specific SNP site (type I / C, type II / A, type III / T, type IV / A) design primers and probes, and cooperate with auxiliary identification. Establish dengue virus SNP typing table, see Table 2 for details.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biomedicine, and relates to a sequencing primer, a detection method and a kit for dengue virus typing. The present invention establishes a multiplex PCR-MS mass spectrometry detection method that comprehensively covers four serotypes of dengue virus and its mutant strains, and is used for laboratory diagnosis, precise genotyping and sample tracing of dengue virus.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a sequencing primer, a detection method and a kit for dengue virus typing. Background technique [0002] Dengue virus (DV) belongs to the genus Flaviviridae of the family Flaviviridae. Dengue virus particles are spherical, with a diameter of 45-55 nm. They are enveloped single-stranded positive-sense RNA viruses. The genome is about 11 kb long. The coding sequence of the entire gene is: 5' I-type cap structure, non-coding region, AUC protein ( Nucleocapsid protein) gene, M protein (membrane protein) gene, E protein (envelope protein) gene, NS1-2a-2b-3-4a-4b-5 gene, non-coding region 3'. There are four serotypes of dengue virus (DENV-1, DENV-2, DENV-3 and DENV-4), all of which can infect humans and cause recessive infection, dengue fever (dengue fever, DF) and dengue hemorrhage Fever (denguehemorrhagicfever, DHF), severe can also lead to dengue shock syndrome (dengueshocksyndrome, DSS),...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6872C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6872C12Q2600/16C12Q2600/156C12Q2531/113C12Q2537/143C12Q2521/525C12Q2533/101C12Q2565/627
Inventor 曹晓梅林健李颖常宇桐张丽萍刘莹莹牛羽李慧鹏
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com