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Space transcriptome position information coding chip and preparation method and application thereof

A technology of location information and spatial transcription, applied in biochemical equipment and methods, combinatorial chemistry, chemical library, etc., can solve the problems of increasing the number of codes and not being able to achieve full coverage of tissue slices, achieving multiple codes and low cost Effect

Pending Publication Date: 2022-01-14
SOUTHEAST UNIV
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Problems solved by technology

However, there are still some areas for improvement in this solution. The first is that the resolution can be improved to the subcellular level. The second is that the number of codes needs to be increased to achieve detection of larger areas. The third is that this technology still cannot achieve full tissue slices. override location marker

Method used

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  • Space transcriptome position information coding chip and preparation method and application thereof
  • Space transcriptome position information coding chip and preparation method and application thereof
  • Space transcriptome position information coding chip and preparation method and application thereof

Examples

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Embodiment 1

[0038] In this example, a spatial position information encoding chip is prepared by preparing a groove imprinting mold and encoding primers by imprinting. We applied the chip to perform spatial transcriptome sequencing in mouse brain slices to evaluate the effect of this technical solution. The specific operation method is as follows:

[0039] 1. Preparation of groove imprinting mold

[0040] Such as figure 1 , choose a groove imprinting mold with a width of 50 μm, which is customized by the manufacturer, and we use 10% PDMS to prepare 4 groups of imprinting molds with 48 groove channels.

[0041] 2. Substrate modification

[0042] The purchased streptavidin is a lyophilized powder with a purity greater than 99% and an activity of 15.7 Units. Use pure water to dissolve the lyophilized powder to a concentration of 5-10 mg / ml. Drops of a streptavidin solution onto a slide or substrate are modified for binding biotin-labeled molecules. see Figure 4 Schematic diagram of sub...

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Abstract

The invention discloses a space transcriptome position information coding chip and a preparation method and application thereof. The space transcriptome position information coding chip comprises a substrate modified by a chemical group and a primer array chip formed by at least two rounds of coding primers connected with the chemical group. According to the spatial transcriptome position information coding chip, full-coverage position marking of tissue slices can be achieved, the spatial resolution is improved, and the scientific research cost is reduced.

Description

technical field [0001] The invention relates to a spatial transcriptome position information coding chip and a preparation method and application thereof, belonging to the field of biotechnology. Background technique [0002] In recent years, the application of single-cell transcriptome sequencing technology has greatly broadened people's horizons, enabling people to deeply understand the diversity of cell composition and gene expression status in tissues. However, the biggest problem is that cells lose their original spatial information in tissues. , which is very important in practical research. Single-cell transcriptome imaging can quantitatively measure the gene expression profile of a cell at a single spatial location and the distribution of transcripts within the cell. For example, spatial transcriptome research is particularly important for revealing some biological functions when studying cell fate mechanisms and information about the spatial location of cell lineag...

Claims

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Application Information

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IPC IPC(8): C40B40/06C40B50/06C12Q1/6869
CPCC40B40/06C40B50/06C12Q1/6869C12Q2535/122
Inventor 葛芹玉贾二腾刘芝余赵祥伟白云飞
Owner SOUTHEAST UNIV
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