Method for simultaneously determining contents of seven antibiotics in water sample
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A technology of antibiotics and content, applied in the field of inspection and analysis, can solve the problems of high cost and slow detection speed, and achieve the effect of low cost, fast detection and accurate results
Pending Publication Date: 2022-01-18
SHENZHEN INST OF TECH
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However, different methods are used for different anti...
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Embodiment 1
[0023] A method for measuring the content of 7 kinds of antibiotics in pharmaceutical enterprise sewage, specifically comprising the following steps:
[0024] 1. Sampling and pretreatment
[0025] In this test, 1L of the water sample from the pharmaceutical sewage treatment pool was taken from the sampling barrel, put into a brown reagent bottle, and the sample was sent to the inspection center immediately. First, use a large-capacity refrigerated centrifuge at 4000rpm, centrifuge for 20min, take the supernatant, and then filter it with a 0.45μm water-based microporous membrane, and immediately place the subsequent filtrate in a refrigerator at 4°C for 12 hours to complete the analysis and detection.
[0026] Because the water samples from sewage pools of pharmaceutical companies contain impurity particles, which will block the solid-phase extraction column, a large-capacity refrigerated centrifuge is used at 4000rpm, centrifuged for 20min, and filtered with a 0.45μm water-bas...
Embodiment 2
[0046] Water samples from sewage pools of Shenzhen cephalosporin antibiotic production and pharmaceutical enterprises were taken, and the contents of 7 cephalosporin antibiotics in the water samples were determined (the spiked amount was 2 μg·L-1). After pretreatment according to the water sample, solid-phase extraction enrichment and ultra-high performance liquid chromatography content analysis were carried out.
[0047]The results showed that the recoveries of ceftizoxime, cefixime, cefathiam, cefazolin, cefodizime, cefuroxime, and cefoxitin all met the trace detection requirements, being 94.3%, 99.2%, and 92.1% respectively. % and 96.8%, 87.5% and 81.8%, 82.1% and 86.8%, 86.2% and 80.9%, 96.4% and 93.0%, 85.8% and 82.6%.
[0048] Visible by above-mentioned embodiment, the method (SPE-UPLC method) of the present invention in conjunction with solid-phase extraction flow process and gradient elution, can detect seven kinds of cephalosporin antibiotics (cefotaxime, cefixime, ce...
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Abstract
The invention provides a method for simultaneously determining the contents of seven antibiotics in a water sample. The method comprises the following steps: centrifuging the water sample, taking the supernate, and conducting filtering to obtain a pre-treated sample; carrying out solid-phase extraction on the pretreated sample through an activated Poly-Sery HLB (Hydrophile-Lipophile Balance) column, so as to obtain an enriched concentrated solution; and performing ultra-high performance liquid chromatography gradient elution on an enriched concentrated solution by taking acetonitrile and 5% acetic acid as mobile phases and taking a chromatographic column as Poroshell 695975-902T EC-C18, and performing ultraviolet spectrum detection to obtain chromatograms of the contents of the seven antibiotics, thereby obtaining the contents of the seven antibiotics. According to the technical scheme, solid-phase extraction and ultra-high performance liquid chromatography are combined, the content of the seven kinds of cephalosporin antibiotics including ceftizoxime, cefixime, cefathiamidine, cefazolin, cefodizime, cefuroxime and cefoxitin can be detected at the same time, detection is rapid, the result is accurate, and the cost is low.
Description
technical field [0001] The invention belongs to the technical field of inspection and analysis, in particular to a method for simultaneously measuring the contents of seven antibiotics in water samples. Background technique [0002] Antibiotics are widely used in the prevention or treatment of bacterial infections in humans and animals. Antibiotics equivalent to tens of billions of unit doses are consumed globally each year. Among them, β-lactam antibiotics such as carbapenems, penicillins, and cephalosporins are currently the most widely used antibiotics. Antibiotics can reach surface and groundwater bodies through sewage from hospitals, aquaculture, and antibiotic production enterprises, posing a major risk to the environment and human health. Due to the low content of antibiotics in the water environment, high content of organic pollutants, and complex components, it is an important link in the effective monitoring of sewage treatment for antibiotic production enterpris...
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