Method for exploring formation time of muscle cells in embryonic period

Abstract

The invention relates to a method for exploring the muscle cell formation time in an embryonic period. The muscle cell formation time is explored by utilizing technologies such as real-time fluorescent quantitative PCR (Polymerase Chain Reaction), flow cytometry, indirect immunofluorescence and the like, and the method is feasible and can be used for massively analyzing the proportion of muscle cells in a plurality of cells in an embryonic disc, and the time when the muscle cells form and expressing marked protein. The method has important guiding significance for researching embryonic disc development and muscle cell formation, establishing a muscle cell formation regulation and control network and the like, and greatly enriches the biological research of embryonic development. The method can be popularized to embryonic plates of different types of poultry in the embryonic period to analyze when various cells in the embryonic plates are formed, differentiated and developed, and a foundation is laid for researching overall embryonic development and decrypting genetic laws. The identification method provided by the invention is simpler, more sensitive and more efficient, can detect weak expression of genes and proteins in an embryonic period, can more truly reflect the formation condition of target cells, and can also be applied to other poultry researches.

Description

technical field [0001] The invention relates to a method for exploring the formation time of embryonic muscle cells, belonging to the field of biotechnology. Background technique [0002] Muscle traits are important traits affecting poultry meat quality. During embryonic myogenesis, skeletal muscle cells can be divided into multiple cellular stages, including embryonic stem cells, somites, myogenic stem cells, myoblasts, and multinucleated myotubes. Muscle fiber is the basic unit of muscle. It is formed by differentiation of embryonic stem cells in the embryonic stage of animals into somite cells and then through a series of proliferation, migration and differentiation. The process of muscle development is mainly divided into two stages: the first stage is the process of myoblast formation, mainly by the migration of somite cells to various parts of the body, and a large number of proliferation, this process is called primary muscle development; the second stage is the proc...

AI Technical Summary

Problems solved by technology

However, it has not been determined at what stage in the chicken embryo these genes first appear and are expressed

Understanding the formation of muscle cells and the expression of key genes plays an important guiding role in the study of meat quality traits. At this stage, the blind spot in the study of embryonic myogenesis limits our application to improve muscle quality at the source as soon as possible, and further exploration and discovery are urgently needed

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