Traditional Chinese medicine imprinting template component cluster classification and integration method based on fingerprint spectrum chromatographic peak matching frequency statistics and product

Abstract

The invention discloses a traditional Chinese medicine 'imprinting template' component cluster incorporation and integration method based on fingerprint spectrum chromatographic peak matching frequency statistics and a product. Based on a fingerprint spectrum chromatographic peak matching frequency statistical principle, according to the characteristics that the retention time of a traditional Chinese medicine fingerprint spectrum chromatographic peak pair is in an imprinting template cluster shape and the matching frequency is distributed in a convex-concave shape, the matching frequency is firstly counted and analyzed, and a traditional Chinese medicine component cluster imprinting template is marked out; a frequency statistical value of retention time between the imprinting templates of each component cluster is calculated, and the imprinting template to which each component cluster belongs is determined; the frequency statistical value of the retention time and other intensity parameters of the imprinting template of each component cluster is calculated to serve as a new retention time and other intensity parameter, and the total peak area and other capacity parameters to serve as a new peak area and other capacity parameters; and the fingerprint spectrum of the traditional Chinese medicine can be converted into a new fingerprint spectrum represented by taking a component cluster 'imprinting template' as a 'unit', so that the total statistical moment characteristic and the inter-sample variability of the original fingerprint spectrum are ensured, and the number of chromatographic peaks can be greatly reduced.

Description

technical field [0001] The invention belongs to the field of biomedicine, and belongs to the interdisciplinary subject combining traditional Chinese medicine and analytical chemistry, and relates to a method for classifying and integrating component clusters of "imprinted templates" of traditional Chinese medicine based on statistics of matching frequency of chromatographic peaks of fingerprints. Background technique [0002] Traditional Chinese medicinal materials, decoction pieces and compound preparations (collectively referred to as products) are the three major material bases of clinical treatment of traditional Chinese medicine. The integration and analysis method of its fingerprint chromatographic peaks is the core key technology to ensure the quality evaluation of traditional Chinese medicine products, and has been highly concerned by the traditional Chinese medicine industry. [0003] 1. The characteristics of the fingerprints of traditional Chinese medicine and the...

AI Technical Summary

Problems solved by technology

[0006] 2. Chinese medicine fingerprint analysis and integrated processing method: due to the complex medicinal components of traditional Chinese medicine and its preparations, multi-level and multi-target points act on some specific links in the biological network of the body according to the supramolecular "imprinting template" to exert its overall At present, traditional Chinese medicine fingerprints are mostly used for quality characterization, control and evaluation, and successively established correlation coefficient method, cluster analysis, angle cosine method, fuzzy sharp T-distribution method, Euclidean distance method, super information feature digitization and summary Quantitative statistical moment (similarity) method, etc., the common feature is that there are many components and chromatographic peaks in traditional Chinese medicine. There are still great difficulties in the theory of Chinese medicine, production and application of Chinese medicine. Therefore, how to integrate the chromatographic peaks of Chinese medicine fingerprints is currently There is no feasible method, resulting in too many and complicated Chinese medicine material basic characterization units, which is not conducive to the research on the theory, production and application of Chinese medicine. Therefore, how to integrate new fingerprints according to the functional characteristics of the "imprinted template" of the component clusters, and establish a "component cluster" "Imprinting template" to represent the basic unit of material without losing the original fingerprint analysis parameter characteristics has become a key "stuck neck" technology in the theory, industry and application research of traditional Chinese medicine, which has attracted the attention of the industry

It would be a breakthrough to solve the problem if we can find an integration method to solve the supramolecular "imprinted template" component clusters of traditional Chinese medicine from the changes in the number of chromatographic peaks and the area of ​​the fingerprint of traditional Chinese medicine.

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