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Application of hsa_circCNOT6_008 in diagnosis of steroid-induced femoral head necrosis

A technology for necrosis of the femoral head and hormones, applied in the field of medicine, can solve the problems of diseased, expensive, and time-consuming

Pending Publication Date: 2022-02-18
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0032] In order to overcome the shortcomings and deficiencies of the prior art: 1. The disease has already occurred when the diagnosis is made; 2. It is expensive and takes a long time; 3. The MRI equipment itself is expensive and cannot be popularized; the purpose of the present invention is to provide a peripheral blood mononuclear cell molecule Target hsa_circCNOT6_008

Method used

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  • Application of hsa_circCNOT6_008 in diagnosis of steroid-induced femoral head necrosis
  • Application of hsa_circCNOT6_008 in diagnosis of steroid-induced femoral head necrosis
  • Application of hsa_circCNOT6_008 in diagnosis of steroid-induced femoral head necrosis

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Embodiment 1

[0055] (1) Screening of candidate targets of circRNAs in peripheral blood mononuclear cells

[0056] 1. Obtaining Peripheral Blood Mononuclear Cells

[0057] The peripheral blood of healthy people and steroid-induced necrosis of the femoral head (ARCO stage IV) requiring total hip arthroplasty (4 cases each) was collected, and then peripheral blood mononuclear cells were extracted. The steps are briefly described as follows:

[0058] (1) Centrifuge at room temperature for 20min (2,000rpm);

[0059] (2) Transfer the cell layer (white) where the monocytes are located to a new tube;

[0060] (3) Add PBS, centrifuge again for 10min (1,500rpm), remove the supernatant, and then add medium (RPMI1640+10%FBS+1%P / S) to perform the same cleaning;

[0061] (4) After the cells are collected, resuspend them with cell freezing solution, put them in a freezer box and place them in a -80°C refrigerator overnight, and transfer the cells to liquid nitrogen for long-term storage the next day; ...

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Abstract

The invention discloses application of hsa_circCNOT6_008 in diagnosis of steroid-induced femoral head necrosis, and belongs to the technical field of medicines. According to the invention, based on an exosome liquid biopsy technology, a new generation sequencing technology NGS is utilized to discover differentially expressed circRNA(s) in peripheral mononuclear cells of a patient with steroid-induced femoral head necrosis; and the differentially expressed circRNA(s) is verified by qRT-PCR (quantitative reverse transcription-polymerase chain reaction) to obtain a potential marker with obvious differential expression, namely the peripheral blood mononuclear molecular target hsa_circCNOT6_008. According to the change of the mononuclear cell molecular target hsa_circCNOT6_008, the steroid-induced femoral head necrosis can be found earlier than MRI (Magnetic Resonance Imaging); the price is low; carrying and popularization are convenient; the area under an AUC curve is 1, and the sensitivity and the specificity are high and both reach 1; the marker is a reliable specific biological marker for diagnosing the steroid-induced femoral head necrosis.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of a molecular target hsa_circCNOT6_008 of steroid-induced necrosis of the femoral head in a diagnostic kit, in particular to a molecular target hsa_circCNOT6_008 of peripheral blood mononuclear cells and its application. Background technique [0002] Steroid-induced necrosis of the femoral head (SONFH) is caused by excessive use of hormones, and is a refractory disease that occurs in young adults aged 30-50. It seriously affects the function of the hip joint and causes hip disability, and is one of the main diseases that cause loss of working ability [1] . Due to the large population base in my country and the irregular use of hormones, the number of high-risk groups of femoral head necrosis is huge and is increasing year by year. It is estimated that there are 200,000 new SONFH patients every year [2] . Obviously, SONFH not only brings a heavy bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113C12N15/11
CPCC12Q1/6883C12Q2600/178C12Q2600/158
Inventor 陈鹏张罡瑜何琪杨均政
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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