Medical application of monascus in preparation of anti-coccidiosis medicine and preparation of monascus
A Monascus, anti-coccidiosis technology, applied to medical preparations containing active ingredients, drug combinations, anti-infectives, etc., to overcome the use of chemical drugs or vaccines, high safety, weight gain and anti-infection Effect
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Embodiment 1
[0013] PDA medium preparation:
[0014] Take 1L of potato juice, add 10g of glucose, and adjust the pH to 6.0. Solid medium needs to add 2% agar. Sterilization conditions: 115°C, 30min.
Embodiment 2
[0016] Potato juice (PDA) medium:
[0017] Take fresh potatoes, peel them, cut them into small pieces, take 200g, add 1L of distilled water, boil on a high heat in an induction cooker, and then time it on a low heat for 1 hour. Then filter with gauze (8 layers), and make up the filtrate to 1L with distilled water, which is the required potato juice.
Embodiment 3
[0019] Inoculate and cultivate Monascus:
[0020] Streak culture of Monascus (AS3.554) on PDA medium, and then use a sterile syringe needle to take 4-5 colonies of Monascus cultured on PDA medium with a size of 1 square centimeter in 500ml of potato juice (PDA) in liquid medium. Shake at 150r / min, culture at 30°C for 7-10 days until the color turns dark red (see attached figure 1 ).
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