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Regulation and control application of m6A demethylase ALKBH5 in gastric cancer cells

A technology of demethylase and gastric cancer cells, applied in the field of regulation and application of gastric cancer cells, can solve the problem of no clear reference standard for the prognosis of gastric cancer, reduce the incidence of adverse prognosis and mortality, improve the efficiency of clinical diagnosis and The effect of prognostic ability

Pending Publication Date: 2022-03-01
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are currently few studies on lncRNAs in gastric cancer, and there is no clear reference standard for predicting the prognosis of gastric cancer.

Method used

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  • Regulation and control application of m6A demethylase ALKBH5 in gastric cancer cells
  • Regulation and control application of m6A demethylase ALKBH5 in gastric cancer cells
  • Regulation and control application of m6A demethylase ALKBH5 in gastric cancer cells

Examples

Experimental program
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Effect test

Embodiment 1

[0082] Example 1. Knockdown of LncRNA PVT1 in zebrafish.

[0083] (1) The gastric cancer cells BGC-823 transfected with si-NC and si-PVT1 were stained with CM-DiI dye (CM-DiI is a lipophilic fluorescent dye that makes the cells show red fluorescence), and the stained cells were collected. cells, approximately 300 cells were injected into the perivitelline space of zebrafish larvae at 48hpf.

[0084] At 1 dpi, juveniles with the same fluorescent area were screened out from all transplanted juveniles using a stereomicroscope.

[0085] Images were taken with a stereomicroscope at 4dpi, including the yolk and torso.

[0086] The quantified fluorescent signal at the yolk represents proliferation, and the quantified fluorescent signal at the trunk represents migration.

[0087] Such as Figure 14 The results showed that the proliferation and migration of gastric cancer cells were inhibited after the expression of LncRNA PVT1 was down-regulated.

[0088] (2) In order to exclude a...

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Abstract

The invention discloses application of m6A demethylase ALKBH5 in regulation and control of gastric cancer cells, expression of LncRNA PVT1 and VEGFA is regulated and controlled at the same time through the m6A demethylase ALKBH5 to inhibit generation of the gastric cancer cells, it is proved that expression of the LncRNA PVT1 and the VEGFA can be regulated and controlled at the same time through the m6A demethylase ALKBH5 to inhibit generation of the gastric cancer cells, early monitoring and effective intervention can be achieved clinically, and the m6A demethylase ALKBH5 can be used for treating gastric cancer cells. According to the present invention, the ALKBH5 and the LncRNA PVT1 can be used as the potential target spot for stomach cancer diagnosis and screening so as to provide the direction for early and accurate stomach cancer prevention, diagnosis and treatment, such that the clinical diagnosis efficiency and the prognosis determination ability of the stomach cancer patient can be further improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the regulation and application of m6A demethylase ALKBH5 in gastric cancer cells. Background technique [0002] Gastric cancer is one of the top three gastrointestinal malignant tumors in the world. The incidence of gastric cancer increases significantly over the age of 40-50. The incidence of gastric cancer is related to factors such as angiogenesis and metastasis in the gastric cancer microenvironment. [0003] Long non-coding RNA (LncRNA) refers to long-chain non-coding RNA, which does not have the function of coding protein, and the length of LncRNA exceeds 200 nucleotide units. Although lncRNAs cannot encode proteins, they can regulate the expression levels of genes and proteins through various pathways, thereby regulating the physiological functions of the body. [0004] In recent years, increasing evidence has shown that many lncRNAs, similar to miRNAs, have altere...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61P35/00C12Q1/6886
CPCA61K38/44A61P35/00C12Q1/6886C12Q2600/158C12Q2600/178C12Q2600/112C12Q2600/118C12Q2600/136
Inventor 蒋华耿茜
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL