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Lactobacillus plantarum capable of producing urate oxidase and inhibiting xanthine oxidase and application of lactobacillus plantarum

A technology of xanthine oxidase and Lactobacillus plantarum, applied in the field of microorganisms, can solve problems such as reducing the level of uric acid, and achieve the effect of significant synergistic effect

Active Publication Date: 2022-03-08
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the Lactobacillus plantarum reported above lowers the uric acid level by inhibiting the activity of xanthine oxidase, and there is no report on the Lactobacillus plantarum that can produce urate oxidase and inhibit xanthine oxidase at the same time.

Method used

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  • Lactobacillus plantarum capable of producing urate oxidase and inhibiting xanthine oxidase and application of lactobacillus plantarum
  • Lactobacillus plantarum capable of producing urate oxidase and inhibiting xanthine oxidase and application of lactobacillus plantarum
  • Lactobacillus plantarum capable of producing urate oxidase and inhibiting xanthine oxidase and application of lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: Isolation and purification of bacterial strains

[0051] 1. Test material

[0052] 1.1 MRS agar medium containing uric acid:

[0053] Glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, ammonium citrate 2g / L, sodium acetate 5g / L, dipotassium hydrogen phosphate 5g / L, manganese sulfate 0.2g / L, Magnesium sulfate 0.5g / L, Tween-80 1mL / L, uric acid 0.2g / L, pH6.0, agar 1.5%, sterilized at 121°C for 30min.

[0054] 1.2MRS agar medium:

[0055] Glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, ammonium citrate 2g / L, sodium acetate 5g / L, dipotassium hydrogen phosphate 5g / L, manganese sulfate 0.2g / L, Magnesium sulfate 0.5g / L, Tween-80 1mL / L, pH 6.0, agar 1.5%, sterilized at 121°C for 30min.

[0056] 1.3 Intestinal contents of laying hens

[0057] Intestinal contents of laying hens obtained from laying hen farm in Huangcheng Town, Zibo, Shandong.

[0058] 2. Strain isolation and purification

[0059] Weigh 10g of the intest...

Embodiment 2

[0062] Example 2: Screening of bacterial strains capable of degrading uric acid

[0063] 1. Reagents

[0064] 1.10.1mol / L sodium phosphate solution: Weigh 38.0g of analytically pure sodium phosphate, add water to dissolve, transfer to a volumetric flask and set the volume to 1L, and adjust the pH to 7.0 with phosphoric acid.

[0065] 1.2 0.2g / L uric acid-0.1mol / L sodium phosphate solution (pH=7.0): Weigh a certain mass of uric acid and dissolve it in 0.1mol / L sodium phosphate solution (pH=7.0) to make the concentration of uric acid 0.2g / L .

[0066] 2. Uric acid detection:

[0067] Uric acid (UA) detection kit (colorimetric method) was purchased from Nanjing Jiancheng Institute of Bioengineering.

[0068] 3. Strain culture

[0069] The nine strains BLCC2-0290, BLCC2-0291, BLCC2-0292, BLCC2-0293, BLCC2-0294, BLCC2-0295, BLCC2-0296, BLCC2-0297, and BLCC2-0298 screened in Example 1 were respectively activated and cultured, Afterwards, the inoculation amount was 4% (volume fr...

Embodiment 3

[0079] Embodiment 3: acid resistance and bile salt resistance test

[0080] 1. Acid resistance test

[0081] Take 10 mL of BLCC2-0296 bacterial suspension fermented for 20 h, centrifuge at 3000 r / min for 15 min, discard the supernatant, wash the bacteria with normal saline for 3 times, first add 5 mL of normal saline, and adjust the suspension of lactic acid bacteria with 10 mol / L hydrochloric acid. The pH values ​​of the solutions were 2.0, 3.0, 4.0, and 6.0, respectively, and then the volume was adjusted to 10 mL with normal saline, and treated at 37 ° C for 2 h. Take the bacterial suspension and count the colonies on the plate, so as to calculate the survival rate of the lactic acid bacteria, and use the bacterial solution with pH 6.0 as the control.

[0082] Survival rate (%) = (the number of viable bacteria in the bacterial liquid to be tested at pH 6.0) × 100%

[0083] 2. Bile salt resistance test

[0084] Get the BLCC2-0296 bacterial suspension of fermentation 20h an...

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Abstract

The invention discloses lactobacillus plantarum capable of producing urate oxidase and inhibiting xanthine oxidase and application of the lactobacillus plantarum, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus plantarum BLCC2-0296 disclosed by the invention is CCTCC (China Center for Type Culture Collection) NO: M 20211236, and the lactobacillus plantarum BLCC2-0296 disclosed by the invention has the advantages that the preservation number is CCTCC NO: M 20211236; the lactobacillus plantarum (Lactobacillus plantarum) BLCC2-0296 disclosed by the invention can be used for producing urate oxidase, and has the capability of directly degrading uric acid; meanwhile, the compound also has xanthine oxidase inhibitory activity, and can significantly improve the effects of reducing uric acid and preventing gout.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum producing uric acid oxidase and inhibiting xanthine oxidase and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Avian gout is a common nutritional and metabolic disease of poultry. The main cause of the disease is excessive production of uric acid due to abnormally active purine metabolism in the body, or excessive uric acid levels in the body due to obstruction of renal excretion of uric acid due to renal dysfunction. Birds are similar to humans. During the evolution of organisms, the urate oxidase gene has mutated, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/06C12P1/04A61K35/747A61P19/06C12R1/25
CPCC12N1/20C12N9/0048C12P1/04A61K35/747A61P19/06
Inventor 谷巍曾佳佳陈静单宝龙翟延庆徐海燕王红陈雷郑军红辛国芹谢全喜纪邑奇兰江华李金鑫崔海英
Owner 山东宝来利来生物工程股份有限公司
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