Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof

A technology for organophosphorus pesticides and microbial inoculants, which is applied in microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, etc., can solve the problem of simultaneous degradation of multiple organophosphorus pesticides by a single microbial strain Promoting growth and preventing diseases, less research on the function of microorganisms, etc., to achieve the effect of inhibiting growth, improving soil environment, and promoting plant growth

Pending Publication Date: 2022-03-18
GANSU ACAD OF SCI INST OF BIOLOGY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) At present, the research on microorganisms degrading organophosphorus pesticides mainly focuses on the establishment of pesticide degradation pathways, mechanisms, metabolites and genetically engineered bacteria, and there are few studies on the functions of microorganisms themselves
[0007] (2) In the prior art, there is no report on the scheme of verifying whether Bacillus subtilis can effectively degrade the organophosphorus pesticides phorate, parathion, chlorpyrifos and trichlorfon
[0008] The difficulty in solving the above problems and defects is: it is difficult to simultaneously degrade multiple organophosphorus pesticides through a single microbial strain, and the degradation rate is above 65%;
[0009] The significance of solving the above problems and defects is: pesticides have made great contributions to the control of crop diseases and insect pests, but pesticide residues have brought serious harm to the environment and human health

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof
  • Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof
  • Microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0047] Such as figure 1 As shown, the preparation method of the microbial bacterial agent with growth promotion, biocontrol and degradation of organophosphorus pesticides provided by the embodiments of the present invention comprises the following steps:

[0048] S101, preparation of microbial slant seeds: inoculate Bacillus subtilis on a bacterial solid slant medium, and cultivate at a constant temperature of 30°C for 48 hours to form a seed slant;

[0049] S102, the cultivation of microbial liquid seeds: inoculate Bacillus subtilis in bacterial liquid culture medium, at a temperature of 30°C, with an initial pH value of 6.0, an inoculum size of 4%, and a rotational speed of 200r / min for 48 hours after shaking and cultivating to form liquid seeds ;

[0050] S103, liquid expansion culture of strains: Inoculate Bacillus subtilis in a 50L fermenter, the culture medium is a bacterial liquid culture medium, the liquid volume is 40L, the temperature is 30°C, the initial pH value i...

Embodiment 1

[0052] Example 1: Microbial agents with growth promotion, biocontrol and degradation of organophosphorus pesticides

[0053] The object of the present invention is to provide a method for biodegrading organophosphorus pesticides. The microorganism has the function of efficiently degrading various organophosphorus pesticides, and can simultaneously promote plant growth, improve soil environment, and inhibit the growth of various plant pathogenic bacteria.

[0054] A microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides. The strain comes from the patent: Bacillus subtilis with high efficiency in degrading organophosphorus and its preparation method, specifically: Bacillus subtilis GSMSC30266, for octyl The degradation rate of phosphorus is 93.5%, and the degradation rate of omethoate is 69.6%.

[0055] The Bacillus subtilis not only has an efficient degradation effect on organophosphorus pesticides phoxim and omethoate, but also has a ...

Embodiment 2

[0076] Example 2: Degradation of Bacillus subtilis to organophosphorus pesticides

[0077] (1) Degradation of organophosphorus pesticide phorate

[0078] According to the patent: Bacillus subtilis with efficient degradation of organic phosphorus and its preparation method, the degradation rate of Bacillus subtilis to phoxim is 93.5%, and the degradation rate to omethoate is 69.6%.

[0079] Add phorate with an active ingredient content of 55% to the inorganic salt medium, so that its addition amount in the medium is 800 μg / mL, inoculate Bacillus subtilis at an inoculation amount of 1%, and place it at a temperature of 30 ° C. Shake culture at 200r / min for 72h, centrifuge at 12000r / min for 10min, take the supernatant, and use the national standard GB / T14552-2003 to determine the residual amount of phorate. The degradation rate of phorate by Bacillus subtilis was 76.3%.

[0080] Add parathion with an active ingredient content of 50% to the inorganic salt medium, so that its add...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of microbial agents, and discloses a microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides and a preparation method, and the microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides comprises bacillus subtilis GSMSC30266. The microbial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides provided by the invention has the functions of efficiently degrading various organophosphorus pesticides, and can promote plant growth, improve soil environment and inhibit the growth of various phytopathogens at the same time. The bacillus subtilis provided by the invention is obtained through 12C6 + ion beam irradiation breeding, has the characteristic of efficiently degrading organophosphorus pesticides, not only can efficiently degrade the organophosphorus pesticides phoxim and omethoate, but also has a relatively good degradation effect on phorate, parathion, chlorpyrifos and trichlorfon, and the degradation rate is 65% or above. The bacillus subtilis microbial agent product disclosed by the invention is simple in production process and has very high application and popularization values.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a microbial bacterial agent capable of promoting growth, biocontrol and degrading organophosphorus pesticides and a preparation method. Background technique [0002] At present, the pollution caused by pesticides to the soil environment, water environment, atmospheric environment and the entire ecosystem is becoming more and more serious, especially the pesticide pollution leads to the accumulation of organisms, which has caused serious harm to human health. More than 80% of organophosphorus pesticides are highly toxic, such as phorate, parathion, methamidophos, etc. In 2003, the Ministry of Agriculture canceled the registration of 5 highly toxic organophosphorus pesticides such as parathion and methamidophos. In 2013, the Ministry of Agriculture promoted the use of chlorpyrifos on vegetables. Low toxicity pesticides such as Baichong are still allo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01N25/32A01N63/22A01P3/00A01P21/00C12R1/125
CPCC12N1/20A01N25/32A01N63/22
Inventor 彭轶楠季彬王治业叶泽赵廷伟梁燕祁宏山
Owner GANSU ACAD OF SCI INST OF BIOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap