Tmem52 protein for regulating glycolipid metabolism, coding gene, sgRNA and application of Tmem52 protein

A technology for glucose and lipid metabolism and protein, applied in the field of medicine, can solve the problems of prohibitive surgery risks, and achieve the effect of regulating abnormal glucose and lipid metabolism and reducing body weight

Active Publication Date: 2022-03-22
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only a small percentage of people can maintain long-term weight loss through diet and exercise. In addition, although the effect of surgical treatment is obvious, the risks of surgery are still prohibitive for many people.

Method used

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  • Tmem52 protein for regulating glycolipid metabolism, coding gene, sgRNA and application of Tmem52 protein
  • Tmem52 protein for regulating glycolipid metabolism, coding gene, sgRNA and application of Tmem52 protein
  • Tmem52 protein for regulating glycolipid metabolism, coding gene, sgRNA and application of Tmem52 protein

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preparation example Construction

[0033]In the present invention, the preparation method of the Tmem52 protein preferably includes: protein cloning and / or tissue extraction; the tissue preferably includes blood and / or pancreas. The method for cloning the protein is not particularly limited in the present invention, and methods well known to those skilled in the art can be used.

[0034] In the present invention, the obesity-related diseases preferably include: one or more of metabolic syndrome, diabetes and fatty liver. The Tmem52 protein provided by the present invention can significantly reduce the body weight of mice, regulate and regulate abnormal glucose and lipid metabolism through the Tmem52 protein provided by the present invention, and can be used for the treatment of obesity and related diseases.

Embodiment 1

[0037] Cloning and purification of the Tmem52 protein consisted of the following steps:

[0038] 1) Acquisition of the target DNA fragment (design primers and amplify the DNA fragment with the sequence shown in SEQ ID NO.2);

[0039] 2) Construct recombinant plasmid;

[0040] Step 1) and step 2) are completed by Gemma Gene;

[0041] 3) introducing the recombinant plasmid into Escherichia coli for expression, purification and identification; the specific steps are as follows:

[0042] Take 100 μL of competent Escherichia coli BL21(DE3), add 4 μL of the recombinant plasmid constructed in step 2) and bathe in ice for 30 minutes; heat shock at 42 °C for 45 s, and bathe in ice for 1 min; add 700 μL of LB medium, shake at 37 °C for 45 min; plate and grow overnight;

[0043] Pick a single colony in 50ml LB liquid medium, shake the bacteria overnight; take 10ml of the bacterial solution and inoculate it into a large bottle containing 1L of LB medium, shake until the OD value is 0.6-...

Embodiment 2

[0048] A sgRNA targeting gene Tmem52 (shown in SEQ ID No.2), the sgRNA is composed of sgRNA-L and sgRNA-R; the nucleotide sequence of the sgRNA-L is shown in SEQ ID No.3, The nucleotide sequence of the sgRNA-R is shown in SEQID No.4.

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Abstract

The invention relates to the technical field of medicines, in particular to a Tmem52 protein for regulating glycolipid metabolism, a coding gene and application of the Tmem52 protein. The amino acid sequence of the Tmem52 protein disclosed by the invention is as shown in SEQ ID NO. 1. According to the invention, C57BL6/n wild mice fed with high-fat feed are taken as research objects, and the weight of the mice can be obviously reduced by utilizing the Tmem52 protein preparation provided by the invention, so that the Tmem52 protein provided by the invention can be used for treating obesity and related diseases by regulating abnormal glucose and lipid metabolism.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to Tmem52 protein, coding gene, sgRNA and application thereof for regulating glucose and lipid metabolism. Background technique [0002] With the continuous improvement of people's living standards, obesity and related diseases such as diabetes, hyperlipidemia, fatty liver, cardiovascular and cerebrovascular diseases and other diseases related to abnormal energy metabolism are now increasingly threatening the health of the Chinese population. According to statistics, the incidence of overweight and obesity among Chinese adults in 2020 is greater than 50%. [0003] Metabolic syndrome is a group of clinical syndromes with central obesity, hyperglycemia, hyperlipidemia, and hypertension, which seriously affect the health of the body. It is characterized by the combination of various metabolic diseases. combination of interrelated risk factors. The pathogenesis of each component is c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12C12N15/113A01K67/027C07K1/14A61K38/17A61P3/04A61P3/00A61P3/10A61P1/16
CPCC07K14/4702C12N15/113A01K67/0276A61P3/04A61P3/00A61P3/10A61P1/16C12N2310/20A01K2227/105A01K2267/0362A61K38/00
Inventor 郑瑞茂刘佳瑞
Owner PEKING UNIV
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