Molecular marker for identifying chicken melanin diffusion gene locus and application of molecular marker in assisted breeding

Abstract

The invention discloses a molecular marker for identifying a chicken melanin diffusion gene locus and application of the molecular marker in assisted breeding, and belongs to the field of molecular marker assisted breeding. The molecular marker is located at the 532bp position of the downstream of an initiation codon of an MC1R gene (GenBank: KF379749.1), and G / A base mutation exists. The polymorphic site gene type related to the feather color is determined through a feather color test cross test and detection of parent and F1 generation MC1R gene site genotypes, and an MC1R gene homozygote strain is established by using a molecular marker-assisted selection method, so that the effects of purifying the pure line chicken MC1R gene and improving the black feather rate of commercial chickens are achieved, and the method can be used for large-scale seed selection and has a wide application prospect. The breeding process is accelerated.

Description

technical field [0001] The invention relates to the field of molecular marker assisted breeding, in particular to a molecular marker for identifying chicken melanin diffusion gene sites and its application in assisted breeding. Background technique [0002] In poultry breeding, feather color has always been an important trait in the breeding of high-quality chickens at home and abroad. To study the relationship between feather color phenotype and genotype can better breed products that meet consumer preferences and meet market demand. At the same time, the feather color gene on the sex chromosome can be used to establish a system for distinguishing between male and female, which greatly improves production efficiency. The melanocortin receptor 1 (MC1R) gene is closely related to the black feather traits of poultry. MC1R is one of the members of the melanocortin receptor family and plays a key role in the pigmentation in vivo. and the main determinant of the distribution. ...

AI Technical Summary

Problems solved by technology

However, in the process of cultivating the breed, it was found through the determination of combining ability that about 7.5% of the Yangtai small black chickens with the best production performance were non-black feather chickens, which affected the consistency of breed traits to a certain extent

There is currently no method for screening black feather feather color genes

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