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Rapid screening kit for genetic genes of congenital heart disease

A congenital heart disease and genetic technology, applied to rigid containers, bottle/can parts, containers, etc., can solve the problems of reducing the rate of gene screening, wasting time, wasting operation time, etc., to achieve diversified use, The effect of speeding up the screening rate and improving the stability

Inactive Publication Date: 2022-03-25
阜外华中心血管病医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the traditional congenital heart disease gene amplifies its DNA samples, it is necessary to place the sample kit and the amplification tube into the wells of the 96-well PCR plate. However, since the pore diameter of the PCR plate is larger than the kit and The outer diameter of the amplification tube, so in the actual placement process, it will cause the kit and the amplification tube to shake in the hole, so when the tester adsorbs the sample and the universal solution inside the DNA sample kit to multiple During the process of increasing the tubes, the stability of the amplification tubes in the wells of the PCR plate is poor, resulting in the inability to precisely drop the sample and the universal solution into the inside of the amplification tubes, wasting a lot of operating time, and causing External pollution and waste of common solutions and samples; in order to accurately drop the sample into the inside of the amplification tube through the quantitative dropper, medical staff usually need to pick up the sample kit with one hand and hold it with the other hand. After the quantitative dropper absorbs the quantitative sample, turn the kit in the hand, take the first amplification tube, drop the quantitative sample absorbed by the quantitative dropper into the first amplification tube, and then Take the kit, put it down after the quantitative dropper absorbs, and then pick up the second amplification tube. When the number of amplification tubes is large, a lot of time is wasted in the frequent reciprocating taking process, which greatly The screening rate of the gene is reduced, and at the same time, it is inevitable to touch other amplification tubes by mistake during the reciprocating taking process, which will cause the risk of the amplification tube being knocked over

Method used

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  • Rapid screening kit for genetic genes of congenital heart disease
  • Rapid screening kit for genetic genes of congenital heart disease
  • Rapid screening kit for genetic genes of congenital heart disease

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Such as Figure 1-10 As shown, the present invention provides a rapid screening kit for congenital heart disease genetic genes, comprising an amplification tube 1, a DNA sample kit 2, and the amplification tube 1 is used to place a sample (such as Figure 7 shown), the DNA sample kit 2 is used to store the DNA sample solution (such as Figure 8 shown), also includes a porous placer assembly 3, the porous placer assembly 3 includes a storage board 31 with a plurality of storage holes 311 on the surface, an anti-fouling box 32 with a top opening, and the size specification of the storage board 31 and a conventional PCR plate The same, and the number and diameter of the storage wells 311 are also the same as those of conventional PCR plate wells.

[0037] The detachable installation between the storage board 31 and the anti-pollution box 32 is convenient for the storage board 31 to be removed from the anti-pollution box 32 for cleaning and reuse. Specifically, L brackets...

Embodiment 2

[0044] Such as Figure 11-13 As shown, the bottom of the fixed pipe 41 is axially provided with a through hole 411, and the inside of the anti-fouling box 32 is provided with a plurality of magnetic separation components 5 that are mated with the through holes 411. The magnetic separation component 5 and the anti-fouling box 32 are detachably installed . To facilitate the disassembly and assembly of the magnetic separation assembly 5 and the anti-pollution case 32, the magnetic separation assembly 5 includes a supporting plate 51 fitted with the internal size of the anti-pollution case 32, installed on the upper surface of the supporting plate 51 and having the same number as the storage holes 311 and A plurality of magnetic pillars 52 on the same axis, the magnetic pillars 52 are adapted to be plugged into the through holes 411 , and the outer wall of the support plate 51 is provided with gaps 511 adapted to the L bracket 321 . Each magnetic column 52 is inserted into the in...

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Abstract

The invention discloses a congenital heart disease genetic gene rapid screening kit which comprises an amplification tube, a DNA sample kit and a porous position object assembly, the porous position object assembly comprises an object placing plate with a plurality of object placing holes formed in the surface and an anti-fouling box with an opening in the top, the object placing plate and the anti-fouling box are detachably installed, and the DNA sample kit is arranged in the anti-fouling box. A gap is reserved between the outer wall of the storage plate and the antifouling box; through the arrangement of the fixing pipes which are detachable from the storage holes, every two fixing pipes are detachable, the DNA sample kit is placed on one side, the multiple amplification pipes are placed on the other side of the DNA sample kit, the multiple amplification pipes and the DNA sample kit can be taken up at a time, frequent taking up is not needed, the amplification pipes are prevented from being knocked over, and the detection efficiency is improved. Meanwhile, through the extrusion plate arranged in the fixed tube, the DNA sample kit and the amplification tube can be firmly fixed in the fixed tube, shaking is prevented, the stability during operation is improved, and the screening rate of detection personnel is increased.

Description

technical field [0001] The invention relates to the field of gene screening for congenital heart disease, in particular to a rapid screening kit for genetic genes of congenital heart disease. Background technique [0002] Congenital heart disease is a birth defect disease with a high incidence in my country, and it is one of the main causes of neonatal death. When the traditional congenital heart disease gene amplifies its DNA samples, it is necessary to place the sample kit and the amplification tube into the wells of the 96-well PCR plate. However, since the pore diameter of the PCR plate is larger than the kit and The outer diameter of the amplification tube, so in the actual placement process, it will cause the kit and the amplification tube to shake in the hole, so when the tester adsorbs the sample and the universal solution inside the DNA sample kit to multiple During the process of increasing the tubes, the stability of the amplification tubes in the wells of the PC...

Claims

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Application Information

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IPC IPC(8): B65D25/10B65D25/02B65D6/24B65D85/20
CPCB65D25/106B65D25/02B65D11/18B65D85/20
Inventor 刘琳王红丹崔存英
Owner 阜外华中心血管病医院
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