Application of glycolytic pathway key enzyme in infantile hemangioma

An infantile hemangioma and key enzyme technology, which is applied in the application field of glycolysis pathway key enzymes in infantile hemangioma, and can solve the problems of non-specificity and poor curative effect.

Pending Publication Date: 2022-03-25
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Description
  • Claims
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Problems solved by technology

In the traditional sense, targeting tumor drivers such as vascular endothelial growth factor (VEGF) and other growth factors to treat tumors is effective at the beginning, but the long-term efficacy is poor because it is not specific. sex

Method used

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  • Application of glycolytic pathway key enzyme in infantile hemangioma
  • Application of glycolytic pathway key enzyme in infantile hemangioma
  • Application of glycolytic pathway key enzyme in infantile hemangioma

Examples

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Embodiment 1

[0053] Inhibition of HemEC proliferation

[0054] The ability of PFK15 to kill HemEC or inhibit the proliferation of HemEC was measured as follows: 24h after PFK15 acted on HemEC, it was detected by CCK8 method. The effect of HemEC being inhibited by PFK15 is as follows: Figure 4A As shown, different concentrations of PFK15 have different inhibitory effects on HemEC, and with the increase of PFK15 concentration, the inhibitory effect on HemEC gradually increases.

[0055] Desired cells are HemEC, containing 8 x 10 in a culture volume of 100 per well 3 Seed in 96 wells. After 24 hours, the cell culture medium was replaced, and the drug was diluted to the desired concentration, mixed in 100 μl medium and added to a 96-well plate to continue interfering with the cells for 24 hours. On the third day, the medium in the 96 wells was aspirated and washed once with PBS, and then 110 μl of CCK8 and medium mixture was added to each well (the volume ratio of CCK8 and medium was 1:10)...

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Abstract

The invention belongs to the technical field of biology, and discloses an application of a glycolytic pathway key enzyme in infantile hemangioma, the glycolytic pathway key enzyme is highly expressed in IH proliferation period tissue and hemangioma endothelial cells, and proliferation and migration of HemEC can be inhibited and apoptosis can be promoted by inhibiting the activity of the key enzyme phosphofructokinase-2/fructose-2, 6-diphosphatase 3. The identification method comprises the following steps: carrying out immunohistochemical analysis on the expression of glycolysis key enzyme in IH proliferation stage tissue and degradation stage tissue, analyzing the expression conditions of HemEC and umbilical vein endothelial cells, and detecting the proliferation, migration and apoptosis capabilities of the HemEC after the inhibitor intervenes in PFKFB3 expression. According to the invention, the expression condition and the effect of a glycolysis pathway key enzyme in IH are determined, the pathological mechanism of IH occurrence and development is disclosed, and an experimental basis is provided for IH treatment taking glycolysis as a target spot.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a key enzyme of glycolysis pathway in infant hemangioma (infantile hemangioma, IH). Background technique [0002] At present, IH is the most common benign tumor in infants and young children, with an incidence rate of about 4-5%. Previous studies have found that differential mRNAs in proliferative and extinction IH tissues are mainly enriched in signaling pathways including metabolic pathways. Studies have shown that glucose metabolism reprogramming, as one of the characteristics of tumor cells, plays an important role in the occurrence and development of various tumors, including lung cancer, breast cancer, and gastric cancer. However, related technical solutions for verifying the role of key enzymes in the glycolysis pathway in IH have not been reported in the prior art. Therefore, it is urgent to design a method that can verify the role of key enzy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573G01N33/531G01N21/64
CPCG01N33/57407G01N33/57484G01N33/573G01N33/531G01N21/6458G01N2333/4703G01N2333/916G01N2333/904G01N2333/91215
Inventor 吉毅杨开颖邱桐张学鹏周江元陈思源龚雪兰钰茹张赓张梓欣
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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