Preparation method of antioxidant camellia seed peptide

A technology for oxidizing camellia oleifera and camellia oleifera seeds, which is applied in the field of preparation of antioxidant camellia oleifera seed peptides, and can solve problems such as insufficient activity of camellia oleifera seed antioxidant peptides, inability to generate economic value, simple processing methods, etc.

Pending Publication Date: 2022-03-29
煌上煌集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For this reason, the present invention aims to solve the problems in the prior art that the traditional processing method is simple, the activity of the prepared Camellia oleifera seed antioxidant peptide is not strong enough, the purity is not high enough, it cannot meet some specific requirements, and it cannot produce high economic value.

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  • Preparation method of antioxidant camellia seed peptide

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preparation example Construction

[0044] see figure 1 , in order to solve this technical problem, the present invention proposes a kind of preparation method of antioxidant camellia oleifera peptide, wherein, said method comprises the following steps:

[0045] Step 1, crushing:

[0046] The tea seed meal is pulverized to 60-100 meshes to obtain tea seed meal powder, and the tea seed meal powder is subjected to degreasing treatment with petroleum ether.

[0047] Step 2, dynamic ultra-high pressure micro-fluidic assisted treatment:

[0048] The tea seed meal powder treated with petroleum ether in step 1 is added with water at a ratio of 1:20 to 30, and the obtained solution is assisted by dynamic ultra-high pressure micro-jet to obtain the first solution.

[0049] In this step, during the process of obtaining the first solution through dynamic ultra-high pressure micro-jet assisted treatment, the treatment time is 40-60 minutes, the treatment temperature is 60-70° C., and the homogeneous pressure is 170-190 MP...

no. 1 example

[0083] The first embodiment of the present invention proposes a preparation method of antioxidant camellia oleifera peptide, which specifically includes the following steps:

[0084] (1) Pulverization: pulverize the tea seed meal to 60-100 meshes, and degrease the tea seed meal powder with petroleum ether;

[0085] (2) Dynamic ultra-high pressure micro-jet assisted treatment: tea seed meal powder was added water at a solid-liquid ratio of 1:25, and the sample was treated with a homogeneous pressure of 175MPa, the treatment time was 50min, and the treatment temperature was 65°C;

[0086] (3) Protein extraction: add NaOH solution to adjust the pH to 10.0, centrifuge to collect the supernatant after leaching for 40min; The conditions are: rotating speed 8000r / min, centrifugation time is 15min;

[0087] (4) High-voltage pulsed electric field treatment: dissolving the Camellia oleifera seed protein solution in water, and treating it for 200 μs at a field strength of 25 kV / cm;

[...

Embodiment 2

[0094] The second embodiment of the present invention proposes a preparation method of antioxidant camellia oleifera peptide, which specifically includes the following steps:

[0095] (1) Pulverization: pulverize the tea seed meal to 60-100 meshes, and degrease the tea seed meal powder with petroleum ether;

[0096] (2) Dynamic ultra-high pressure micro-jet assisted treatment: tea seed meal powder was added with water at a solid-liquid ratio of 1:30, and the sample was treated with a homogeneous pressure of 180MPa, the treatment time was 50min, and the treatment temperature was 67°C;

[0097] (3) Protein extraction: add NaOH solution to adjust the pH to 10.0, centrifuge to collect the supernatant after leaching for 40min; The conditions are: rotating speed 8000r / min, centrifugation time 15min;

[0098] (4) High-voltage pulsed electric field treatment: dissolving the Camellia oleifera seed protein solution in water, and treating it for 200 μs at a field strength of 25 kV / cm; ...

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Abstract

The invention provides a preparation method of antioxidant camellia seed peptide, which comprises the following steps: crushing camellia seed meal to obtain camellia seed meal powder, and degreasing with petroleum ether; adding water into the tea seed meal powder, and performing dynamic ultrahigh-pressure micro-jet assisted treatment to obtain a first solution; adding a NaOH solution into the first solution, extracting, centrifuging and collecting to obtain supernate; adding hydrochloric acid into the supernate to precipitate protein, centrifuging and precipitating to obtain a camellia seed protein solution; dissolving the camellia seed protein solution in water, and performing high-voltage pulse treatment under a high-voltage pulse electric field; performing multi-step enzyme reaction on the camellia seed protein solution to obtain camellia seed peptide; d-fructose and camellia seed peptide are weighed and subjected to glycosylation treatment in a high-temperature steam environment; phenolation modification: enrichment of peptides; and carrying out spray drying on the obtained antioxidant camellia seed peptide to obtain a finished product.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of antioxidant camellia oleifera peptide. Background technique [0002] Active peptide (English Active Peptide) is a general term for more than one thousand peptides (such as soybean peptide, deep-sea fish skin peptide, sea cucumber peptide, etc. are one of the active peptides). Peptides are compounds in which two or more amino acids are connected by peptide bonds, which play important physiological roles in the human body and exert physiological functions. Active polypeptides are called active peptides, also known as biologically active peptides or biologically active polypeptides. [0003] Among them, antioxidant peptides are a very important class of active peptides, which can delay lipid oxidation in the body and prevent active oxygen from damaging cells and tissues. In addition, free radicals are easy to accumulate in the human body, which may ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A23J3/14A23J3/34A23J1/14
CPCA23J3/14A23J3/346A23J1/14
Inventor褚剑涂宗财郭德斌王辉刘光宪胡月明陈海奇邱丽聪李军
Owner煌上煌集团有限公司