Improved buffer for sperm capacitation

Abstract

A buffer solution for sperm capacitation is composed of a buffer solution comprising an active ingredient astaxanthin combined with serum albumin and a phosphate buffer solution, and the phosphate buffer solution is composed of monopotassium phosphate KH2PO4 buffered by dipotassium phosphate K2HPO4.

Description

[0001] Background of the invention [0002] The present invention relates to improved buffers for the capacitation of spermatozoa. [0003] The field of the invention is that of medically assisted fertilization techniques, in which spermatozoa are collected in a medium, also called buffer, composed of nourishing and protective fluids that must guarantee their viability and function composition. In particular, due to its optimized properties, in said buffer it is possible to select the most motile sperm (by the swim-up technique), but above all to "mature", ie to complete a series of modifications without which they would not be able to acquire their fertilization capabilities. [0004] The problem found in commercial buffers, such as, for example, the well-known Pure Sperm Wash buffer (PSW from Nidacon), is mainly that it does not allow the sperm to reach a sufficient level of capacitation for fertilization, which has been analyzed and described as one of the best buffers on ...

AI Technical Summary

Problems solved by technology

These studies were carried out by extemporaneous addition of astaxanthin to the medium containing spermatozoa at the time of incubation, therefore the time evolution of biochemical parameters and motility related to the denaturation of the product due to the instability of the final composition was not investigated any investigation

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