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Lactobacillus fermentation culture medium and process for producing lactic acid at high yield

A lactic acid bacteria fermentation and culture medium technology, applied in the direction of microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of high production cost and low acid production, and achieve the effect of prolonging the growth time and increasing the production of lactic acid

Inactive Publication Date: 2015-11-25
沈阳科纳提克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The use of lactic acid bacteria to ferment and produce lactic acid requires some special nutritional components due to the high nutritional requirements for the growth of lactic acid bacteria; at the same time, lactic acid bacteria secrete organic acid metabolites during the growth process, which makes the pH value in the culture solution drop continuously. When the pH value When it drops below 4.10, the growth and reproduction of the bacteria will be inhibited, and acid production will stop at the same time. Therefore, the traditional production method of strains has low acid production and high production costs.

Method used

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  • Lactobacillus fermentation culture medium and process for producing lactic acid at high yield
  • Lactobacillus fermentation culture medium and process for producing lactic acid at high yield
  • Lactobacillus fermentation culture medium and process for producing lactic acid at high yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 Fermentation medium and technology production lactic acid in the present invention

[0048] Bacteria: Leuconostoc Mesenteroides

[0049] Anaerobic bottle seed medium formula (weight and volume percentage): peptone: 10g / L, beef extract: 10g / L, yeast extract powder: 5g / L, glucose: 20g / L, sodium acetate: 5g / L, citric acid Ammonium: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate heptahydrate: 0.1g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80: 1g / L, and the balance is water. The pH value was adjusted to 7.0 with 2mol / L sodium hydroxide.

[0050] Seed tank medium formula (weight and volume percentage): peptone: 10g / L, beef extract: 10g / L, yeast extract powder: 5g / L, glucose: 20g / L, sodium acetate: 5g / L, ammonium citrate: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate heptahydrate: 0.1g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80: 1g / L, and the balance is water. The pH value was adjusted to 7.0 with 2mol / L sodium hyd...

Embodiment 2

[0059] Embodiment 2 Fermentation medium and process production lactic acid in the present invention

[0060] Bacteria: Leuconostoc Mesenteroides

[0061] Anaerobic bottle seed medium formula (weight and volume percentage): peptone: 10g / L, beef extract: 10g / L, yeast extract powder: 5g / L, glucose: 20g / L, sodium acetate: 5g / L, citric acid Ammonium: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate heptahydrate: 0.1g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80: 1g / L, and the balance is water. The pH value was adjusted to 7.0 with 2mol / L sodium hydroxide.

[0062] Seed tank medium formula (weight and volume percentage): peptone: 10g / L, beef extract: 10g / L, yeast extract powder: 5g / L, glucose: 20g / L, sodium acetate: 5g / L, ammonium citrate: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate heptahydrate: 0.1g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80: 1g / L, and the balance is water. The pH value was adjusted to 7.0 with 2mol / L sodium hydrox...

Embodiment 3

[0069] Embodiment 3 Fermentation medium and process production lactic acid in the present invention

[0070] Bacteria: Leuconostoc Mesenteroides

[0071] Anaerobic bottle seed medium formula (weight and volume percentage): peptone: 10g / L, beef extract: 10g / L, yeast extract powder: 5g / L, glucose: 20g / L, sodium acetate: 5g / L, citric acid Ammonium: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate heptahydrate: 0.1g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80: 1g / L, and the balance is water. The pH value was adjusted to 6.5 with 2mol / L sodium hydroxide.

[0072] Seed tank medium formula (weight and volume percentage): peptone: 10g / L, beef extract: 10g / L, yeast extract powder: 5g / L, glucose: 20g / L, sodium acetate: 5g / L, ammonium citrate: 2g / L, dipotassium hydrogen phosphate: 2g / L, magnesium sulfate heptahydrate: 0.1g / L, manganese sulfate monohydrate: 0.05g / L, Tween-80: 1g / L, and the balance is water. The pH value was adjusted to 6.5 with 2mol / L sodium hydrox...

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Abstract

The invention relates to the field of lactobacillus fermentation and particularly relates to a lactobacillus fermentation culture medium and a process for producing lactic acid at high yield. The lactobacillus fermentation culture medium is composed of peptone, a beef extract, a yeast extract or yeast extract powder, glucose, sodium acetate, ammonium citrate, dipotassium phosphate, magnesium sulfate heptahydrate, manganese sulfate monohydrate, tween-80, inulin and water. Compared with the existing process for producing lactic acid through microbial fermentation, the process disclosed by the invention can be used for remarkably (P<0.05) prolonging the growth time of lactobacillus and remarkably (P<0.01) increasing the yield of lactic acid.

Description

technical field [0001] The invention relates to the field of lactic acid bacteria fermentation, in particular to a lactic acid bacteria fermentation medium and a high-yield production process for lactic acid. Background technique [0002] Leuconostoc Mesenteroides is an important strain of the genus Leuconostoc in lactic acid bacteria, also known as flavor bacteria, aroma bacteria and aroma-producing bacteria. Studies have confirmed that Leuconostoc enterococcus can ferment sugars to produce a variety of acids and alcohols, and has high acid production capacity, antioxidant capacity and the ability to antagonize pathogenic bacteria. At present, Leuconostoc enterolis has been widely used in flavoring agents and plasma substitutes, and is expected to become a new type of microecological preparation. [0003] Lactic acid is one of the three recognized organic acids in the world, and it has a wide range of uses. Lactic acid and its salts and derivatives can be used in industri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/01
Inventor 李平顺姜黎黎王宏丽张朔胡紫嫣
Owner 沈阳科纳提克生物科技有限公司
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