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Willow bark extract as well as compound preparation and application thereof

A technology of willow bark extract and compound preparations, which can be used in pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., and can solve the problems of induction, NDM strain stimulation, strong drug resistance, etc.

Pending Publication Date: 2022-04-05
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term use of antibiotics may stimulate or induce stronger drug resistance of NDM strains, almost breaking through the last line of defense in clinical anti-infection treatment

Method used

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  • Willow bark extract as well as compound preparation and application thereof
  • Willow bark extract as well as compound preparation and application thereof
  • Willow bark extract as well as compound preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Extraction and identification of a super drug-resistant strain of NDM-5-producing porcine Escherichia coli

[0032] 1. Materials

[0033] 1.1 Source of strain

[0034] 87 strains of bacteria with reduced susceptibility to imipenem isolated from cases of diarrhea in piglets in the farrowing room were kept in our laboratory, and the 87 strains were screened by using the drug-sensitive disk method to obtain 1 strain producing New Delhi metal β-endo Amidase (NDM-5) positive strain, the strain is named: Escherichia coli, preserved in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC No.23303.

[0035] 1.2 Reagents and equipment

[0036] Soybean casein agar medium, soybean casein medium, physiological saline, incubator, refrigerator, pipetting gun, alcohol lamp, plate, test tube, etc.

[0037] 2. Method

[0038] 2.1 Identification of strains

[0039] The strains were identified by amplification and sequencing of Esch...

Embodiment 2

[0065] For the screening of the antibacterial Chinese medicine extract of the NDM-5 type porcine Escherichia coli super drug-resistant strain of producing NDM-5 of embodiment 1

[0066] 1. Extract Preparation

[0067] 1.1 Preparation of traditional Chinese medicine extract

[0068] Select the combined liquid of water extracts and / or ethanol extracts of 11 traditional Chinese medicines such as honeysuckle, forsythia, viola, dandelion, isatidis, shegan, mountain bean root, xanthophyll, baiyaozi, andrographis paniculata, and willow bark, and recover ethanol and concentrate the extract. Specifically:

[0069] Weigh 100g of each traditional Chinese medicine, add 8 times the amount of water to soak for 3 hours, heat and boil, change to slow fire decoction for 0.5 hours, then filter, collect the supernatant, add 6 times the amount of 65% ethanol to the residue, heat and boil , changed to slow fire decoction, filtered, combined the supernatants of the two extractions, recovered eth...

Embodiment 3

[0100] Determination of Compound Preparation Containing Willow Bark Extract

[0101] 1. Determination of compound preparations

[0102] 1.1 Determination of the formula of compound preparation in vitro

[0103] The willow bark extract with a concentration of 1 g / mL of the original drug was mixed with penicillin G, ampicillin, hetacillin, amoxicillin, oxacillin, cloxacillin, cephalothin, and cephalexin in β-lactam drugs, respectively. , cefadroxil, cefamandole, cefoxitin, cefaclor, cefuroxime, cefotaxime, ceftizoxime, ceftriaxone, cefoperazone, ceftazidime, ceftiofur, cefbizime, cefquinome 21 kinds of β-lactam antibiotics such as joint application, carry out agar diffusion test to the NDM-5 type porcine Escherichia coli super drug-resistant bacteria, observe whether there is an inhibition zone, and measure its diameter, the specific method is shown in the first paragraph of embodiment 2 2 bars.

[0104] 1.2 Determination of the formulation of the compound preparation in vivo...

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Abstract

The invention provides a willow bark extract as well as a compound preparation and application thereof. The concentration of a raw medicine in the willow bark extract is 1g / mL, and a solvent is water or an ethanol-water mixed solution. The willow bark extract can be used for preparing a beta-lactamase producing bacterial inhibitor or bactericide, and the willow bark extract and antibiotics are combined for use, so that the willow bark extract has a very good synergistic bacteriostatic effect on NDM-5 type swine escherichia coli, and is expected to be further developed into an effective bacteriostatic agent or bactericide of the super-drug-resistant strain.

Description

technical field [0001] The invention belongs to the technical field of antibacterial drug development, and in particular relates to a willow bark extract, its compound preparation and application. Background technique [0002] Piglet diarrhea in farrowing house has always been a serious digestive tract disease that harms piglets in farrowing house in pig production. In intensive and large-scale pig farms, small and medium-scale pig farms, and rural individual pig farms, piglet diarrhea in farrowing rooms is a common disease in pig production. This disease not only leads to slow growth of piglets, low feed remuneration, inconsistent body size, and feeding Ratio rises, become a dead pig or even die, and the mortality rate is high, can reach 100% mortality rate. Seriously affected the operating conditions of large-scale pig farms, brought great harm to the pig industry and caused major economic losses. The main causes of diarrhea in piglets in the farrowing room are as follow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/76A61K45/06A61P31/04A61K129/00
Inventor 李海利郎利敏徐引弟张青娴游一王治方朱文豪张立宪焦文强方剑玉王克领刘运平许峰
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
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