Test strip for joint detection of fungaltoxin as well as preparation method and application of test strip
A technology for joint detection and mycotoxins, applied in biological testing, measuring devices, material inspection products, etc., to achieve the effect of sensitive joint detection
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Embodiment 1
[0028] This embodiment provides a test strip for joint detection of mycotoxins, the structure of which is as follows: figure 1 As shown, it includes a support plate, a glass cellulose membrane, a nitrocellulose membrane, an absorbent pad, and a sample pad, wherein the support plate is a strip structure and is located at the bottom, and the sample pads are sequentially arranged above the support plate , the glass cellulose membrane, the nitrocellulose membrane and the water-absorbent pad; the nitrocellulose membrane is sequentially provided with deoxynivalenol, zearalenone toxin, Aspergillus flavus B1, fumonisins, T2 Toxin, ochratoxin detection line and quality control line; the glass nitrocellulose membrane contains quantum dot-labeled deoxynivalenol antibody, quantum dot-labeled zearalenone toxin antibody, quantum dot-labeled Aspergillus flavus B1 antibody , quantum dot-labeled fumonisin antibody, quantum dot-labeled ochratoxin antibody, quantum dot-labeled T2 toxin antibody ...
Embodiment 2
[0031]This embodiment provides a method for preparing a test strip for joint detection of mycotoxins, which specifically includes the following steps:
[0032] S1, preparation of quantum dot-labeled DON antibody: take 0.02M, pH = 6.1 MES buffer 100ul, mix and add to 10ul quantum dot microsphere particles with a concentration of 100mg / ml, and then add sodium carboxymethylcellulose 100mg, add the activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, after activating for 15min, add β-mercaptoethanol, the final concentration is 5mM, add lysine, the final concentration To 10mM, react for 15min.
[0033] According to the mass ratio of DON antibody to quantum dot microspheres: 1:20, add DON antibody to quantum dot microspheres, mix well for 3 hours, centrifuge at 8000rpm for 20min, and resuspend the precipitated particles with blocking solution , mixed at room temperature for 1 hour, then centrifuged at 8000rpm for 20 minutes, and the precipitated particles were red...
Embodiment 3
[0040] This embodiment provides a method for preparing a test strip for joint detection of mycotoxins, which specifically includes the following steps:
[0041] S1, preparation of quantum dot-labeled DON antibody: take 0.02M, PH=6.5 MES buffer 100ul, mix and add to 10ul of quantum dot microsphere particles with a concentration of 100mg / ml, and then add sodium carboxymethylcellulose 100mg, add the activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, after activating for 15min, add β-mercaptoethanol, the final concentration is 5mM, add lysine, the final concentration To 10mM, react for 15min.
[0042] According to the mass ratio of DON antibody to quantum dot microspheres of 1:20, add DON antibody to the quantum dot microsphere particles, mix well for 3 hours, centrifuge at 15000rpm for 10min, and resuspend the precipitated particles with blocking solution. Mix at room temperature for 1 hour, then centrifuge at 15,000 rpm for 10 minutes, and redissolve the pre...
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Abstract
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