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Test strip for combined detection of HCG and P as well as preparation method and application of test strip

A technology of combined detection and test strips, which is applied in the field of test strips for combined detection of HCG and P, can solve the problems of not detecting HCG kits at the same time, and achieve the effect of sensitive combined detection

Pending Publication Date: 2022-04-26
北京易斯威特生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, during pregnancy testing, it is necessary to detect both substances at the same time, but at present, most pregnancy test strips can only detect HCG, and there is no kit for simultaneous detection of HCG and P

Method used

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  • Test strip for combined detection of HCG and P as well as preparation method and application of test strip
  • Test strip for combined detection of HCG and P as well as preparation method and application of test strip

Examples

Experimental program
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Embodiment 1

[0032] This embodiment provides a test strip for combined detection of HCG and P, the structure of which is as follows: figure 1 As shown, it includes a support plate, a glass cellulose membrane, a nitrocellulose membrane, an absorbent pad and a sample pad, wherein the support plate is a strip structure and is located at the bottom, and the sample pads are sequentially arranged above the support plate , the glass cellulose membrane, the nitrocellulose membrane and the water-absorbing pad; the nitrocellulose membrane is sequentially provided with HCG detection line, P detection line and quality control line; the glass nitrocellulose membrane contains There are quantum dot-labeled HCG antibody, quantum dot-labeled P antibody and quantum dot-labeled IgY.

[0033] In the structure of the test strip in this embodiment, the top of the sample pad presses and sticks to the bottom of the glass cellulose membrane, and the top of the glass cellulose membrane presses the nitrocellulose me...

Embodiment 2

[0035]This embodiment provides a method for preparing a test strip for combined detection of HCG and P, which specifically includes the following steps:

[0036] S1, preparation of quantum dot-labeled HCG antibody: Take 100ul of 0.02M MES buffer solution with pH=6.1, mix it into 10ul of quantum dot microsphere particles with a concentration of 100mg / ml, and then add 100mg of sodium carboxymethylcellulose, Add the activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, after activating for 15min, add β-mercaptoethanol, the final concentration is 5mM, add lysine, the final concentration is 10mM , react for 15min.

[0037] According to the mass ratio of HCG antibody to quantum dot microspheres of 1:20, add HCG antibody to the quantum dot microspheres, mix for 3 hours, centrifuge at 8000rpm for 20 minutes, resuspend the precipitate particles with blocking solution, and mix for 1 hour at room temperature , and then centrifuged at 8000rpm for 20min, the precipitated ...

Embodiment 3

[0044] This embodiment provides a method for preparing a test strip for combined detection of HCG and P, which specifically includes the following steps:

[0045] S1, preparation of quantum dot-labeled HCG antibody: take 100ul of 0.02M MES buffer solution with pH=6.5, mix it into 10ul of quantum dot microsphere particles with a concentration of 100mg / ml, and then add 100mg of sodium carboxymethylcellulose, Add the activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, after activating for 15min, add β-mercaptoethanol, the final concentration is 5mM, add lysine, the final concentration is 10mM , react for 15min.

[0046] According to the ratio of HCG antibody to quantum dot microsphere mass ratio of 1:20, add HCG antibody to the quantum dot microsphere particles. After mixing for 3 hours, centrifuge at 15,000 rpm for 10 minutes, resuspend the precipitate particles with blocking solution, and mix at room temperature for 1 hour. , and then centrifuged at 15,000rp...

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Abstract

The invention discloses a test strip for combined detection of HCG and P. The test strip comprises a supporting plate, a glass cellulose membrane, a nitrocellulose membrane, a water absorption pad and a sample pad, the supporting plate is of a long-strip structure and located at the bottommost part, and the sample pad, the glass cellulose membrane, the nitrocellulose membrane and the water absorption pad are sequentially arranged above the supporting plate; an HCG detection line, a P detection line and a quality control line are arranged on the nitrocellulose membrane; the glass nitrocellulose membrane comprises a quantum dot labeled HCG antibody, a quantum dot labeled P antibody and a quantum dot labeled chicken IgY. In the scheme, a mixture of an HCG antibody marked by quantum dots and a P antibody marked by quantum dots is coated on a glass cellulose membrane by using a sodium carboxymethyl cellulose system to serve as a detection probe, and a fluorescence immunochromatography method is realized by combining the principles of a double-antibody sandwich method and a competition method with the fluorescence characteristic of the quantum dots; meanwhile, rapid, specific, simple, convenient and sensitive combined detection is carried out on HCG and P.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a test strip for combined detection of HCG and P, a preparation method and an application thereof. Background technique [0002] Human chorionic gonadotropin (HCG) and progesterone (P) are key hormones secreted during pregnancy, among them, human chorionic gonadotropin (HCG) is a glycoprotein secreted by the trophoblast cells of the placenta, which is Consists of glycoproteins of alpha and beta dimers. A glycoprotein hormone with a molecular weight of 36700, the α subunit is basically similar to FSH (follicle stimulating hormone), LH (luteinizing hormone) and TSH (thyroid-stimulating hormone) secreted by the pituitary gland, so they can cross-react with each other, while the β subunit The base structures vary. In mature women, after fertilized eggs move to the uterine cavity and implant, embryos are formed. During the development and growth of fetuses, placental sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/76G01N33/74G01N33/558G01N33/548G01N33/58G01N33/533
CPCG01N33/76G01N33/743G01N33/558G01N33/548G01N33/588G01N33/582G01N33/533G01N2800/36
Inventor 林涛
Owner 北京易斯威特生物科技股份有限公司