Modeling and evaluation method for mice with depression and liver depression fire syndrome

Abstract

The invention discloses a modeling method for mice suffering from depression and liver depression fire syndrome diseases, and relates to the field of animal model construction methods, the modeling method comprises the following steps: performing continuous sleep deprivation stimulation on healthy mice for 2-30 days, and simultaneously performing light irradiation daily stimulation and next-day grain stimulation every day, the obtained model mouse has symptomatic manifestation of depression, and meanwhile, the characteristic that the model has the syndrome of liver depression transforming into fire is judged through a traditional Chinese medicine syndrome diagnosis method. The depression mouse model established by the invention not only has clinical liver depression transforming into fire and depression states in general signs, but also can be used for treating depression through weight, total distance of open field activities, time, times and average speed of entering the center of the open field, times and time of entering an open arm in an elevated cross labyrinth, water intake and forced swimming immobile time. The model accords with the characteristics of liver depression transforming into fire in traditional Chinese medicine, and can be used for further research on pathogenesis and treatment of the syndrome of liver depression transforming into fire of depression.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for making a model of depression mice with liver stagnation and fire syndrome. Background technique [0002] Depression is a serious mental illness, mainly manifested as low mood, loss of interest, slow thinking and other psychological abnormalities. The World Health Organization predicts that by 2030, depression will rise to the top of the global disease burden. [0003] At present, only 20-30% of depression patients are effective to drug treatment, and some patients will stop taking medicine due to unbearable adverse reactions. Therefore, it is imminent to explore effective and safe antidepressant drugs. The pathogenesis of depression is complex, and its specific mechanism is not fully understood. Although some limitations have been overcome by using postmortem brain samples from human subjects, these samples are often influenced by factors such as ...

AI Technical Summary

Problems solved by technology

So far, the animal models of different TCM syndrome types of depression mainly include animal models of TCM "syndrome" established on the basis of Western medicine disease models. On the basis of the disease, artificial intervention may change the original Western medicine disease animal models As a result, it is difficult to meet the requirements of animal models for both "disease" and "syndrome"; many animal models of TCM "syndrome" are caused by adverse reactions produced by western medicine

Images