Homogeneous test micro-fluidic chip and detection system

Abstract

The homogeneous test micro-fluidic chip comprises a main body, a first liquid storage cavity, a first quantitative cavity, a mixing cavity, a first flow channel and a first capillary tube are arranged on the front surface of the main body, and a second liquid storage cavity, a second quantitative cavity, a waste liquid cavity, a second capillary tube, a second flow channel and an overflow channel are arranged on the back surface of the main body; the first liquid storage cavity is communicated with the first quantitative cavity through a first flow channel, the first quantitative cavity is communicated with the mixing cavity through a first capillary tube, the second liquid storage cavity is communicated with the second quantitative cavity through a second flow channel, the second quantitative cavity is communicated with the waste liquid cavity through an overflow channel, and the first quantitative cavity is communicated with the waste liquid cavity through a first through hole. The second capillary tube is communicated with the mixing cavity through the second through hole. A sample and a diluent flow on the front side and the back side, so that automatic operation and control of each detection process are realized, the detection process is more convenient and efficient, the whole detection process can be realized on a smaller main body, the size of a chip is effectively reduced, and the cost is reduced.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a homogeneous test microfluidic chip and a detection system. Background technique [0002] Chemiluminescence immunoassay can be divided into heterogeneous chemiluminescence method and homogeneous chemiluminescence method according to whether there are separation and washing steps. The homogeneous chemiluminescence method is based on the short-distance diffusion of two nanometer microspheres using single-line oxygen energy to stimulate the chemiluminescent reaction of the adjacent sites that have been formed to measure the interaction between biomolecules. It is non-radioactive and consists of Proximity binding of biomolecules on capture microspheres results in energy transfer from one microsphere to another, through chemical reactions and ultimately the generation of a luminescent signal. Heterogeneous chemiluminescence relies on physical separation and also requires was...

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Problems solved by technology

[0004] Based on this, it is necessary to provide a homogeneous test microfluidic chip and detection system, which can effectively reduce the size of the chip and reduce the cost, in order to solve the problems of large size and high cost of the traditional microfluidic chip used for homogeneous chemical analysis.

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