A method for making a 3D printing bio-ink material with controllable distribution of materials and a method for making a three-dimensional biomimetic hydrogel scaffold

Abstract

A method for manufacturing a 3D printing bio-ink material with controllable distribution of materials and a method for preparing a three-dimensional biomimetic hydrogel scaffold. The controllable distribution of materials involves the controllable distribution of patterns and components of materials, and includes the following steps: S1, 3D Print a customized high-precision patterned mold that fits with the inner surface of the 3D printer barrel as a template for regulating material distribution; S2 prepares thermo-induced hydrogels and cold-induced hydrogels with high concentration and temperature-sensitive properties. The resulting hydrogel was used as a mold surface coating material; S3 prepared a bioink material with controllable distribution of materials. The present invention utilizes the coating material and combines with the method of inserting the mold—casting the outer layer of cold-induced hydrogel—released—casting the inner layer of cold-induced hydrogel, so as to well realize the personalized customization and high-precision control of material distribution. , and endowed the 3D printed heterogeneous scaffold with good 3D structure. In addition, combined with cellular biochemical factors, the alginate-based hydrogel scaffolds can be further endowed with the cellular regulation ability.

Description

technical field [0001] The invention belongs to the technical field of biological 3D printing, and in particular relates to a preparation method of a 3D printing biological ink material with controllable distribution of materials and a preparation method of a three-dimensional biomimetic hydrogel stent. Background technique [0002] At present, many complex human organs in the clinic lack clinically available substitutes. The tissue engineering method adopts the addition of scaffolds, growth factors and cells to the culture medium for in vitro synthesis, or implants scaffolds and growth factors into the body for synthesis, which provides a way for tissue and organ repair. 3D printing technology adopts the idea of ​​discrete / stacking, which can produce entities with arbitrarily complex internal structures, which can not only realize the preparation of different material compositions and complex structures on the same slice, but also the composition and structural form on diff...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that traditional tissue engineering scaffolds mainly focus on providing temporal and spatial position resolution for cell growth, and it is difficult to regulate cell behavior and prepare functional tissues, and provide a 3D printed biological material with controllable distribution. Ink material production method and three-dimensional biomimetic hydrogel scaffold preparation method, this method realizes the personalized customization and high-precision control of material distribution through customizing molds, introducing coating materials and demolding, and endows 3D printing with unique features. Good three-dimensional structure of the scaffold

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