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Method for collecting hepatic portal vein blood of machin

A technology of hepatic portal vein and cynomolgus monkey is applied in the field of collecting hepatic portal vein blood of cynomolgus monkey, which can solve the problems of large animal damage, high requirements on consumables and high difficulty in operation, and achieve the effects of less animal damage, simple operation and low cost.

Pending Publication Date: 2022-05-17
苏州方达新药开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method of collecting blood from the hepatic portal vein is difficult to operate, causes great harm to animals, and requires high consumables

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for collecting hepatic portal vein blood of cynomolgus monkeys, comprising the following steps:

[0026] S1. Preoperative preparations;

[0027] The preoperative preparations include hair shaving, physical examination, anesthesia, establishment of venous access and connection to a breathing anesthesia machine, etc.

[0028] S2. Cut along the midline of the abdomen of the animal, taking the navel as the base point to cut up and down;

[0029] Specifically, cut about 2.2-2.8 cm up and down along the midline of the abdomen of the animal, taking the navel as the base point.

[0030] S3. After the abdominal cavity is exposed, the jejunum segment of the animal is gently pulled out of the abdominal cavity;

[0031] S4. Separate a vein on the mesentery of the jejunum segment, ligate the distal end, clamp the proximal end with a vein clamp, and then pass through the vein with a silk thread;

[0032] S5. Cut a small opening at the distal end of the vein, insert the ca...

Embodiment 2

[0045] A method for collecting hepatic portal vein blood of cynomolgus monkeys, comprising the following steps:

[0046] S1. Preoperative preparations;

[0047] The preoperative preparations include hair shaving, physical examination, anesthesia, establishment of venous access and connection to a breathing anesthesia machine, etc.

[0048] S2. Cut along the midline of the abdomen of the animal, taking the navel as the base point to cut up and down;

[0049] Specifically, cut about 2.2-2.8 cm up and down along the midline of the abdomen of the animal, taking the navel as the base point.

[0050] S3. After the abdominal cavity is exposed, the jejunum segment of the animal is gently pulled out of the abdominal cavity;

[0051] S4. Separate a vein on the mesentery of the jejunum segment, ligate the distal end, clamp the proximal end with a vein clamp, and then pass through the vein with a silk thread;

[0052] S5. Cut a small opening at the distal end of the vein, insert the ca...

Embodiment 3

[0065] A method for collecting hepatic portal vein blood of cynomolgus monkeys, comprising the following steps:

[0066] S1. Preoperative preparations;

[0067] The preoperative preparations include hair shaving, physical examination, anesthesia, establishment of venous access and connection to a breathing anesthesia machine, etc.

[0068] S2. Cut along the midline of the abdomen of the animal, taking the navel as the base point to cut up and down;

[0069] Specifically, along the midline of the abdomen of the animal, cut about 2.4-2.6 cm up and down with the navel as the base point.

[0070] S3. After the abdominal cavity is exposed, the jejunum segment of the animal is gently pulled out of the abdominal cavity;

[0071] S4. Separate a vein on the mesentery of the jejunum segment, ligate the distal end, clamp the proximal end with a vein clamp, and then pass through the vein with a silk thread;

[0072] S5. Cut a small opening at the distal end of the vein, insert the cath...

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PUM

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Abstract

The invention discloses a method for collecting hepatic portal vein blood of cynomolgus monkeys, and relates to the technical field of blood collection. Comprising the following steps: preoperative preparation work; cutting up and down along the middle abdominal line of the animal by taking the navel as a base point; after the abdominal cavity is exposed, the jejunum section of the animal is slightly pulled out of the abdominal cavity; a vein on the mesentery of the jejunum section is separated, the telecentric end is ligated, the proximal end is clamped by a vein clamp, and then a silk thread penetrates through the vein; a small opening is cut in the distal end of the vein, a catheter is inserted, after the catheter is inserted into the vein by 0.8-1.2 cm, a silk thread placed in advance is knotted, and the vein clamp is removed; the catheter is continuously inserted by 7-8 cm, and whether the catheter reaches the hepatic portal vein or not is detected by going deep into the abdominal hepatic portal vein with the hand; the catheter is fixed; resetting the ileum, leading the catheter out of the abdominal cavity, and closing the abdominal cavity; a small opening is formed in the abdominal wall on the right side, and the catheter is connected with the Port and buried subcutaneously.

Description

technical field [0001] The invention relates to the technical field of blood collection, in particular to a method for collecting hepatic portal vein blood of cynomolgus monkeys. Background technique [0002] Portal blood collection in cynomolgus monkeys is a commonly used technique in preclinical pharmacokinetic studies. However, the current method of collecting blood from the hepatic portal vein is difficult to operate, causes great harm to animals, and has high requirements for consumables. Contents of the invention [0003] The purpose of the present invention is to provide a method for collecting hepatic portal vein blood in cynomolgus monkeys, so as to solve the problems raised in the above-mentioned background technology. [0004] To achieve the above object, the present invention provides the following technical solutions: [0005] A method for collecting hepatic portal vein blood of cynomolgus monkeys, comprising the following steps: preoperative preparation; ...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61B5/15A61B5/153
CPCA61D1/00A61B5/15003A61B5/153A61B2503/40A61B2503/42
Inventor 黄常桂
Owner 苏州方达新药开发有限公司
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