Primer of molecular marker related to cauliflower color and method for identifying cauliflower color

Abstract

The invention provides a primer of a molecular marker related to cauliflower color, the primer comprises a forward primer FAM, a forward primer HEX and a reverse primer C, the nucleotide sequence of the primer FAM is as shown in SEQ ID NO: 1, the nucleotide sequence of the primer HEX is as shown in SEQ ID NO: 2, and the nucleotide sequence of the reverse primer C is as shown in SEQ ID NO: 3. The invention also provides a method for identifying the color of the cauliflower, and the method comprises the following steps: extracting the genome DNA of the leaves of the cauliflower, carrying out PCR amplification by taking the genome DNA of the leaves of the cauliflower as a template to obtain a PCR amplification product, analyzing the PCR amplification product, determining that the cauliflower with the allele AGT inserted in the genome position interval 50387046bp-50387048bp is the cabbage flower, and the yellow cauliflower with the allele AGT deleted in the genome position interval 50387046bp is the yellow cauliflower. The method can realize rapid auxiliary identification of cauliflower color, and is high in efficiency and reliable in result compared with a traditional field test.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to a primer for molecular markers related to the color of cauliflower and a method for identifying the color of cauliflower. Background technique [0002] Molecular marker is a genetic marker technology based on the polymorphism of biological macromolecules. With the advent of molecular marker technology, indirect selection in plant breeding has been achieved, significantly improving the effectiveness of breeding and the accuracy of genetics. Therefore, the status of molecular marker technology in the field of genetic breeding is increasing. Molecular labeling technology includes two types, namely isoenzyme labeling and molecular labeling (ie, DNA labeling). Among them, due to the particularity of post-translational modification, developmental stage, tissue specificity and few polymorphic sites, it is difficult to realize the identification technology of isoz...

AI Technical Summary

Problems solved by technology

This kind of classic molecular marker technology has some complicated experimental steps, low repeatability, and limitations of probe factors, etc., which makes DNA templates highly demanding and in large demand, resulting in low final polymorphism detection efficiency.

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