Method for expanding propagation of arbuscular mycorrhizal fungus inoculant in degenerated sandy soil

Abstract

The invention discloses a propagation method of a degenerated sandy soil arbuscular mycorrhizal fungus inoculant, and relates to the technical field of production of microbial inoculants. Comprising the following steps: preparing a culture medium: sterilizing substrate raw materials, and uniformly mixing to prepare the culture medium; (2) accelerating germination of the host plant: accelerating germination of the sorghum hybrid sudangrass seeds to obtain germinated sorghum hybrid sudangrass seeds; and (3) inoculation and propagation: inoculating arbuscular mycorrhizal fungi in the culture medium, then sowing the germinated sorghum hybrid sudangrass seeds, removing the overground part when the sorghum hybrid sudangrass grows to a harvest time, air-drying the residual root system and the medium part, crushing and uniformly mixing to obtain the degenerated desertified soil arbuscular mycorrhizal fungi inoculant. The sorghum hybrid sudangrass is used as a host plant, and a large number of arbuscular mycorrhiza can be obtained in a short time; the special arbuscular mycorrhizal fungus inoculant propagation method and technology are established for the degenerated sand wasteland, and technical guidance and application foundation are provided for ecological restoration of the degenerated sand wasteland.

Description

technical field [0001] The invention relates to the technical field of production of microbial inoculants, in particular to a propagation method of arbuscular mycorrhizal fungal inoculants in degraded desertified soil. Background technique [0002] Mycorrhiza is a mutualistic symbiosis formed by fungi and plant roots, which is a common plant symbiosis in nature. The distribution of mycorrhizal fungi is global, from the North Pole to the Antarctic of the earth. Involves farmland crops, wild plants, polar alpine plants, tropical plants, halophytes, etc. Arbuscular mycorrhizal fungi (AMF) are endophytic mycorrhizal fungi and one of the oldest asexual fungi in existence. Arbuscular mycorrhizal fungi exist in a variety of habitats in the ecosystem. Many studies have shown that arbuscular mycorrhizal fungi have significant effects on plant nutrient absorption and utilization (especially phosphorus), drought resistance, cold resistance, salinity resistance, and heavy metal poison...

AI Technical Summary

Problems solved by technology

The advantage of this method is that the fungus and the substrate are easy to separate, and a large amount of pure bacterial agent can be obtained, but the technology is difficult, and it is only used for small-scale inoculation and reproduction in scientific research work, and cannot be mass-produced

[0013] Since arbuscular mycorrhizal fungi are symbiotic microorganisms, they cannot be cultured in vitro, and the acquisition of high-purity arbuscular mycorrhizal fungal agents requires special culture equipment and professional technicians. The cultivation costs are high, the operation is complicated, and mass production is impossible; There are many kinds of mycorrhizal fungi, each of which has its unique biological characteristics and characteristics. Most of the prior art is a general propagation method; the method of mixing other substrates with soil is often used to treat arbuscular mycorrhizal fungi. Propagation cannot avoid the influence of the external environment on arbuscular mycorrhizal fungi; moreover, the existing propagation method requires a special propagation device, and the operation cost is high

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