Preparation method and application of melanoma autologous tumor vaccine for high expression of ADAM-28

An ADAM-28, melanoma technology, applied in the field of vaccines, to achieve the effect of improving the expression level and obvious tumor immune prevention effect

Active Publication Date: 2022-06-03
SUZHOU HEALIRNA BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are very few studies on the relationship between ADAM-28 and melanoma.

Method used

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  • Preparation method and application of melanoma autologous tumor vaccine for high expression of ADAM-28
  • Preparation method and application of melanoma autologous tumor vaccine for high expression of ADAM-28
  • Preparation method and application of melanoma autologous tumor vaccine for high expression of ADAM-28

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Embodiment 3

[0020] image 3 For the relationship between tumor volume and time, it can be seen that the tumor growth rate of the B16-Adam28 group is significantly lower than that of the other groups, and the difference is significant; the results show that the melanoma autologous tumor vaccine with high ADAM-28 expression disclosed in the present invention has a very good effect. Good tumor immune preventive effect.

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Abstract

The invention provides a preparation method and application of a melanoma autologous tumor vaccine with high expression of ADAM-28. ADAM-28 genes are amplified from B16 cells through a PCR method, the ADAM-28 genes are cloned into a lentiviral vector to construct recombinant lentivirus, B16-F10 cells are infected with the recombinant lentivirus to obtain a melanoma cell line with high expression of ADAM-28, and after irradiation inactivation, the melanoma autologous tumor vaccine with high expression of ADAM-28 is obtained. The melanoma autologous tumor vaccine is obtained. According to the invention, the melanoma cell line with high expression of ADAM-28 is prepared for the first time, and the melanoma autologous tumor vaccine is prepared from the melanoma cell line. A mouse tumor model verifies that the vaccine efficiently activates CD8 + T cell immune response and has an obvious tumor immunoprophylaxis effect, so that the vaccine can be applied to the field of preparation of tumor immunoprophylaxis drugs, especially melanoma.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to the preparation and application of a melanoma autologous tumor vaccine highly expressing ADAM-28. Background technique [0002] Melanoma is a skin cancer caused by malignant transformation of melanocytes. It is mainly caused by ultraviolet (UV)-induced DNA mutation. It mostly occurs in the skin, but occasionally in mucous membranes and internal organs. It has the characteristics of high invasiveness and easy recurrence. . Melanoma is the third most common skin cancer, and the incidence of melanoma is rising rapidly and is one of the cancers with the highest incidence. Among them, metastatic melanoma is highly lethal and is the most deadly skin cancer. Immune system mainly through CD8 + T cells mediate cytotoxic T lymphocyte (CTL) effects to kill tumor cells. The T cell response process depends on the directional movement of cells and immune recognition. Integrins are importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00C12N15/57C12N15/867C12N5/10
CPCA61K39/001158A61K39/00119A61P35/00C12N9/6491C12N15/86C12N5/0693A61K2039/5156A61K2039/876C12N2740/15043C12N2510/00
Inventor 赵李祥冯天云易诚葛友桢胡洪鹏蒋剑豪钟天翼
Owner SUZHOU HEALIRNA BIOTECHNOLOGY CO LTD
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