Cell culture device

Abstract

The invention provides a cell culture apparatus. A cell culture device according to an exemplary embodiment of the present invention comprises: a housing having a plurality of through-holes formed on at least one surface thereof so as to allow carbon dioxide to flow from the outside, and having an internal space filled with a culture medium for cell culture; a plurality of plate-shaped supports which are disposed in the internal space at intervals in multiple stages so as to facilitate cell culture and which have a prescribed area; and a porous member which is attached to one surface of the housing so as to cover the plurality of through-holes, prevents the culture medium filled in the internal space from leaking to the outside, and allows the carbon dioxide to flow into the internal space from the outside.

Description

technical field [0001] The present invention relates to a cell culture apparatus, and more particularly, to a medium-set cell culture apparatus that can be used in a cell culture system in which the medium is not circulated during cell culture. Background technique [0002] Cell culture is a method of culturing and proliferating in a container by giving nutrients to tissue sections excised from an individual of a multicellular organism. [0003] In the rapidly developing field of life engineering after the 1980s, as animal cell culture technology played an important role, from the mid-1980s, the importance of large-scale animal cell culture technology began to become prominent. [0004] Animal cells derived from human or animal tissue can be cultured by floating in the medium or attached to a carrier. Cells mainly derived from blood cells (including hematopoietic stem cells) are floating cells, and cells derived from tissues such as skin, liver, or lung, and embryonic stem ...

AI Technical Summary

Problems solved by technology

[0005] Therefore, when scaling up (scale-up), buoyant cells are favored in order to maintain the highest cell density per unit volume

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