Preparation method of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel and application of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel in skin inflammation repair

A technology for recombining collagen and Pichia pastoris, applied in skin diseases, skin care preparations, medical preparations containing active ingredients, etc., can solve the problems of damage to the body, difficult side effects for patients, etc., and achieve low production costs, Good skin wound repair effect, high porosity effect

Pending Publication Date: 2022-06-07
湖南有美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patients are often difficult to be cured by conventional means, and traditional treatment drugs have large side effects and damage the body

Method used

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  • Preparation method of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel and application of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel in skin inflammation repair
  • Preparation method of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel and application of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel in skin inflammation repair
  • Preparation method of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel and application of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel in skin inflammation repair

Examples

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Effect test

Embodiment 1

[0029] A preparation method of tea polyphenol-Pichia pastoris recombinant collagen sponge scaffold gel, which comprises the following steps:

[0030] (1) Preparation of mixture: Mix the Pichia pastoris recombinant collagen solution and the Pichia pastoris recombinant collagen sponge scaffold protective agent alkyl glucoside evenly, add tea polyphenols and mix evenly to prepare a mixture. In this example, the concentration of the Pichia recombinant collagen solution is 5 mg / g, the mass ratio of the Pichia recombinant collagen solution to tea polyphenols is 20:1, and 0.1% alkyl glucoside is used as the Pichia recombinant Collagen protector.

[0031] Wherein, the preparation method of Pichia pastoris recombinant collagen solution is as follows: inoculating recombinant Pichia pastoris engineering bacteria in a culture medium to obtain a crude extract, then eluting the crude extract, and collecting the eluate; the eluate is sequentially subjected to desalting, After the purificati...

Embodiment 2

[0036] A preparation method of tea polyphenol-Pichia pastoris recombinant collagen sponge scaffold gel, which comprises the following steps:

[0037] (1) Preparation of mixture: Mix the Pichia pastoris recombinant collagen solution and the Pichia pastoris recombinant collagen sponge scaffold protective agent alkyl glucoside evenly, add tea polyphenols and mix evenly to prepare a mixture. In this example, the concentration of the Pichia recombinant collagen solution was 7 mg / g, the mass ratio of the Pichia recombinant collagen solution to tea polyphenols was 19:1, and 0.3% alkyl glucoside was used as the Pichia recombinant Collagen protector.

[0038] Wherein, the preparation method of Pichia pastoris recombinant collagen solution is as follows: inoculating recombinant Pichia pastoris engineering bacteria in a culture medium to obtain a crude extract, then eluting the crude extract, and collecting the eluate; the eluate is sequentially subjected to desalting, After the purific...

Embodiment 3

[0043] A preparation method of tea polyphenol-Pichia pastoris recombinant collagen sponge scaffold gel, which comprises the following steps:

[0044] (1) Preparation of mixture: Mix the Pichia pastoris recombinant collagen solution and the Pichia pastoris recombinant collagen sponge scaffold protective agent alkyl glucoside evenly, add tea polyphenols and mix evenly to prepare a mixture. In this example, the concentration of the Pichia recombinant collagen solution is 10 mg / g, the mass ratio of the Pichia recombinant collagen solution to tea polyphenols is 18:1, and 0.5% alkyl glucoside is used as the Pichia recombinant Collagen protector.

[0045] Wherein, the preparation method of Pichia pastoris recombinant collagen solution is as follows: inoculating recombinant Pichia pastoris engineering bacteria in a culture medium to obtain a crude extract, then eluting the crude extract, and collecting the eluate; the eluate is sequentially subjected to desalting, After the purificatio...

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Abstract

The invention relates to the technical field of sponge scaffolds, in particular to a preparation method of tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel and application of the tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel in skin inflammation repair. The preparation method of the tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel comprises the following steps: (1) preparing a mixture; (2) preparing a stent; and (3) preparing the gel. According to the preparation method, pichia pastoris recombinant collagen is used as a drug carrier to construct the tea polyphenol-pichia pastoris recombinant collagen sponge scaffold, and the prepared tea polyphenol-pichia pastoris recombinant collagen sponge scaffold has the advantages of high interconnectivity and porosity, so that drug release is not influenced, and the preparation method is simple and convenient. The skin inflammation repairing effect is good. The alkyl glucoside has a good protection effect on the pichia pastoris recombinant collagen. The tea polyphenol-pichia pastoris recombinant collagen sponge scaffold gel disclosed by the invention is outstanding in effect, mild, non-irritant, stable and effective for a long time, and suitable for inhibiting and relieving skin inflammation reactions caused by various reasons such as eczematous dermatitis, atopic dermatitis, hormone-dependent dermatitis, xerodermia, sensitive skin, acne, laser treatment and the like; the traditional Chinese medicine composition can be used for promoting wound healing and skin repair, shortening the course of disease, relieving hyperpigmentation after inflammation and reducing the risk of scar formation, and has a certain auxiliary treatment effect on the diseases.

Description

technical field [0001] The invention belongs to the field of cosmetic products, and particularly relates to a preparation method of a tea polyphenol-Pichia pastoris recombinant collagen sponge scaffold gel for external use and its application in skin inflammation repair. Background technique [0002] Skin diseases are now a major problem affecting human health, among which skin inflammatory diseases and tumor diseases are the most representative. Skin inflammation can lead to a variety of skin diseases, such as dermatitis eczema, atopic dermatitis, hormone-dependent dermatitis, dry skin, sensitive skin, acne, etc. Patients are often difficult to be cured by conventional means, and traditional treatment drugs have large side effects and damage the body. [0003] Tea polyphenols can inhibit TPA-induced inflammation, ODC activity, proliferation and hydrogen peroxide formation, topical tea polyphenols can inhibit TPA-induced skin inflammation in mice, and can resist UV damage t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9728A61K8/04A61K8/49A61K8/65A61P17/00A61P29/00A61Q19/00
CPCA61K8/9728A61K8/65A61K8/498A61K8/042A61Q19/00A61P17/00A61P29/00A61K2800/84
Inventor 戈欣蔡少红
Owner 湖南有美生物科技有限公司
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