Preparation method and application of 2'-deoxyguanosine, guanosine and composition thereof

A technology of deoxyguanosine and a composition is applied in the field of preventing and treating pathogenic microorganisms, which can solve the problems of complex composition and inability to determine the antiviral components in the strain extract, and achieve excellent antiviral effect, enhanced immunity, Realize the effect of industrialized production

Active Publication Date: 2022-08-02
山东蓬勃生物科技有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent document CN110178857A (application number: 201910575412.0) discloses the use of a fermented extract of seabuckthorn endophytic fungus strain SJ1. The comp

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of 2'-deoxyguanosine, guanosine and composition thereof
  • Preparation method and application of 2'-deoxyguanosine, guanosine and composition thereof
  • Preparation method and application of 2'-deoxyguanosine, guanosine and composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Preparation of extracts from Paecilomyces variotii SJ1 cells

[0070] The Paecilomyces varostii SJ1 strain was connected to the flat PDA medium, cultured at 25 °C for 6 days, and the agar block with bacterial cells was inoculated into a 250 mL Erlenmeyer flask with 50 mL PDA medium. ℃, at 120 r / min for 3 days on a rotary shaker as the seed solution, inoculate the seed solution at 10% volume into a 500 mL conical flask containing 150 mL of PDA medium, and at 28 ℃, 120 r / min at 28 ℃. The fermentation was terminated by incubating on a rotary shaker for 5 days. The cultured mycelium was washed and dried at 60 °C, weighed, then pulverized by a high-speed pulverizer, and passed through a 60-mesh sieve. After mixing, 1200 W ultrasonic treatment was performed for 60 min, vacuum filtration, and the filtrate was collected for use, and the filtrate was the extract of Paecilomyces varostii SJ1 strain.

Embodiment 2

[0072] Take 1 mL of the extract of Paecilomyces varostii SJ1 strain prepared in Example 1 into a 1.5 mL centrifuge tube, and centrifuge at 12,000 r / min for 10 min. 10 compounds were obtained by semi-preparative chromatography gradient elution separation, and 10 compound samples were taken respectively. See the liquid chromatogram and compound number. figure 1 .

Embodiment 3

[0074] Example 2 Antiviral test of 10 compounds isolated from the extract

[0075] (1) Tobacco seedling raising: Tobacco seedling raising is carried out according to the conventional seedling raising method.

[0076] (2) Inoculation and application method: after the tobacco grows to 4-8 leaves, the tobacco seedlings are obtained, and the tobacco with the same growth tendency is selected to use the 100 ng / mL concentration of 200 ng / mL, and the dosage of 10 mL of 10 mL. Each compound sample was sprayed on tobacco leaves, five tobacco trees were sprayed for each treatment, and sprayed with water as a control, each treatment was repeated 3 times, pay attention to spraying each leaf evenly, and the method of friction inoculation after two hours of treatment Inoculate three true leaves under the leaves of tobacco system, take 50 μL of virus solution and inoculate gently on the front of each true leaf with 600-mesh quartz sand. The viruses inoculated are tobacco mosaic virus (TMV) an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method and application of 2'-deoxyguanosine, guanosine and compositions thereof, belonging to the technical field of prevention and control of pathogenic microorganisms, in particular to the inhibition of parahemolysis by 2'-deoxyguanosine and guanosine in marine fish culture The application of vibrio, the application of 2'-deoxyguanosine and guanosine in anti-plant virus, and the application of 2'-deoxyguanosine and guanosine in improving 2'-deoxyguanosine, guanosine, salicylic acid in plants The application in the content; the present invention provides a composition comprising 2'-deoxyguanosine and guanosine, and provides a kind of extracting 2'-deoxyguanosine, guanosine from Paecilomyces variotans SJ1 cells The present invention finds that 2'-deoxyguanosine and guanosine have the effect of preventing and controlling pathogenic microorganisms, especially have obvious anti-plant virus effects, and can improve the 2'-deoxyguanosine, guanosine, saliculus in plants acid content.

Description

technical field [0001] The invention belongs to the technical field of preventing and treating pathogenic microorganisms, in particular to a preparation method and application of 2'-deoxyguanosine, guanosine and compositions thereof. Background technique [0002] Plant virus diseases are known as "plant cancers", which can cause serious plant diseases and cause huge economic losses to agricultural production. At present, the control methods of plant virus diseases mainly include: agricultural control, such as detoxification of seedlings, rational crop rotation, selection of disease-resistant varieties, etc.; chemical pesticide control, common control pesticides include guanidine copper acetate, amino oligosaccharide, lentinan, Morpholine guanidine hydrochloride, etc. In the actual prevention and control of plant virus diseases, chemical control is still the main method, but there is no effective chemical pesticide to control plant virus diseases. The development of chemica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N43/90A01P1/00A01P21/00C07H19/173C07H19/167C07H1/06C02F1/50C02F103/20
CPCA01N43/90C07H19/173C07H19/167C07H1/06C02F1/50C02F2103/20
Inventor 王庆彬向亚美路冲冲孙健王胜孔波王洪凤张晓英彭春娥赵红玲
Owner 山东蓬勃生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap