Antihypertensive peptide as well as preparation method and application thereof
A blood pressure-lowering peptide and source technology, applied in the field of biomedicine, can solve the problems of hydrophobic amino acids that have not been reported in relevant research, and achieve excellent antihypertensive effect, high inhibitory activity, and excellent effect
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Embodiment 1
[0049] Example 1. Preparation of corn antihypertensive peptide
[0050] S1. Ultrasonic pretreatment of zein: add deionized water to zein at a ratio of 1:100, seal it and place it in an ultrasonic tank at 26°C, control the ultrasonic frequency to 28kHz, the ultrasonic power to 60W, and the ultrasonic The time is 50min;
[0051] S2. Enzymatically hydrolyze the pretreated zein in an isopropanol-water system to obtain an enzymatic hydrolysis product: take the ultrasonically treated zein, add 60v / v% isopropanol-10v / v% ethanol -In the water system, after shaking at 55℃ to dissolve, add pepsin for enzymolysis, enzymolysis conditions: substrate mass concentration 3w / v%, pH 8.5, adding enzyme amount 1.5w / v%, enzymolysis Temperature 45℃, enzymolysis for 1.5h;
[0052] S3. Separating, purifying, and freeze-drying the enzymatic hydrolysis product to obtain a blood pressure-lowering peptide: the enzymatic hydrolysis is terminated in a water bath at 85° C. for 5 minutes, and after coolin...
Embodiment 2
[0053] Example 2. Preparation of corn antihypertensive peptide
[0054] S1. Ultrasonic pretreatment of zein: add deionized water to zein at a ratio of 1:120, seal it and place it in an ultrasonic bath at 26°C, control the ultrasonic frequency to be 40kHz, the ultrasonic power to be 60W, and the ultrasonic The time is 30min;
[0055] S2. Enzymatically hydrolyze the pretreated zein in an isopropanol-water system to obtain an enzymatic hydrolysis product: take the ultrasonically treated zein, add 50v / v% isopropanol-12v / v% ethanol -In the water system, shake it at 55°C to dissolve it, then add pepsin for enzymatic hydrolysis, enzymatic hydrolysis conditions: substrate mass concentration 1.5w / v%, pH 8.0, adding enzyme amount 2w / v%, enzymatic hydrolysis Temperature 50 ℃, enzymatic hydrolysis 2h;
[0056] S3. Separating, purifying, and freeze-drying the enzymatic hydrolysis product to obtain a blood pressure-lowering peptide: the enzymatic hydrolysis is terminated in a water bath...
Embodiment 3
[0057] Example 3. Preparation of corn antihypertensive peptide
[0058] S1. Ultrasonic pretreatment of zein: add deionized water to zein at a ratio of 1:150, seal it and place it in an ultrasonic bath at 26°C, control the ultrasonic frequency to be 32kHz, the ultrasonic power to be 80W, and the ultrasonic The time is 45min;
[0059] S2. Enzymatically hydrolyze the pretreated zein in an isopropanol-water system to obtain an enzymatic hydrolysis product: take the ultrasonically treated zein, add 70v / v% isopropanol-10v / v% ethanol -In the water system, shake it at 55°C to dissolve it, then add pepsin for enzymatic hydrolysis. Enzymatic hydrolysis conditions: substrate mass concentration 5% w / v, pH 9.0, amount of enzyme added 1% w / v, enzyme The hydrolysis temperature was 52 °C, and the enzymatic hydrolysis was carried out for 3 hours;
[0060] S3. Separating, purifying, and freeze-drying the enzymatic hydrolysis product to obtain a blood pressure-lowering peptide: the enzymatic...
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