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Bacillus velezensis and fermented feed thereof for degrading microbial toxins and application of bacillus velezensis

A bacillus and microorganism technology, which is applied in the application field of various microorganisms fermenting and degrading DON, Bacillus velesi and its fermented feed to degrade microbial toxins, can solve problems such as the harm of toxin concentration, and achieve the improvement of flavor and palatability. Effect

Pending Publication Date: 2022-07-05
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the adsorbents are not selective, and nutrients such as vitamins and trace elements in the feed will also be adsorbed. At the same time, the combination of adsorbents and vomitoxin is not effective in the intestinal tract of animals under the influence of digestive enzymes or intestinal microorganisms. Stable, which may lead to the re-release of vomitoxin in the intestinal tract of animals, and sometimes even cause the local toxin concentration to increase sharply and cause harm

Method used

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  • Bacillus velezensis and fermented feed thereof for degrading microbial toxins and application of bacillus velezensis
  • Bacillus velezensis and fermented feed thereof for degrading microbial toxins and application of bacillus velezensis
  • Bacillus velezensis and fermented feed thereof for degrading microbial toxins and application of bacillus velezensis

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Experimental program
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Effect test

Embodiment 1

[0092] Mutagenesis screening and identification of Bacillus velesi BL-14 degrading DON

[0093] 1. Screening of strains

[0094] (1) Steps of ARTP mutagenesis

[0095] ① The strain FBL-4 was inoculated onto the LB medium from the slant for activation, and a single colony was picked and placed in a 5ml LB liquid medium test tube, incubated at 37°C, 180rpm shaker for 12h, and then transferred to a 50ml LB liquid shaker flask, 37 ℃, 180rpm shaking culture for 6-8h.

[0096] ②Pump 1ml of bacterial solution into a 2ml EP tube, centrifuge at 8000rpm for 10min, pour off the culture medium to leave the cell pellet, add sterile distilled water, shake the EP tube to resuspend the cellulite, centrifuge again at 8000rpm for 10min, pour off the supernatant, repeat 2 ~3 times, add sterile distilled water to the precipitate, resuspend it into a bacterial suspension, and adjust the concentration of the bacterial suspension with sterile distilled water to make the OD value around 0.8.

[00...

Embodiment 2

[0109] Degradation of DON, Aflatoxin B1 and Zearalenone in Feed Ingredients by Anaerobic Fermentation

[0110] (1) Bacillus velesi BL-14 (CGMCC No. 22083) was transferred from a slanted preservation test tube to a LB medium plate, and cultured at 37°C for 12 hours; Lactobacillus plantarum RS-1 (GCMCC No. 1.2158) and Bacillus coagulans RS-10 (GCMCC No. 1.6565) was plated on MRS medium from slant preservation tubes.

[0111] (2) Pick a single colony of BL-14 strain into LB liquid medium (50 mL), and cultivate at 37°C and 180 rpm shaker for 12 hours; pick a single colony of RS-1 and RS-10 strains into MRS liquid medium (50 mL) ) in a constant temperature biochemical incubator at 42°C for 24 hours.

[0112] (3) Mix corn germ meal, corn starch residue, shotcrete corn husk and water in advance.

[0113] (4) Mix the bacterial liquid of strains BL-14, RS-1 and RS-10 in a ratio of 3:1:1, add 6% of the inoculum to the pre-mixed fermented material, and put it into the fermented material ...

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Abstract

The invention relates to bacillus velezensis BL-14 which is subjected to mutagenesis screening and a liquid mixed bacterium solution of the bacillus velezensis BL-14, wherein the liquid mixed bacterium solution is used for efficiently degrading deoxynivalenol (DON), aflatoxin (AFB1) and zearalenone (ZEN) in feed raw materials. The strain BL-14 (CGMCC No.22083) has a rich hydrolase system, can produce amylase, protease, cellulase and the like, can be combined with lactobacillus plantarum 1.2158 (CGMCC) and bacillus coagulans 1.6565 (CGMCC) to form a mixed bacterial agent for feed raw material fermentation, can degrade 83.25% of vomitoxin, 90.09% of aflatoxin (AFB1) and 80.76% of zearalenone in the raw materials, has the lactic acid content of 36.5 g / Kg in the fermented feed, and has the advantages that the lactic acid content of the fermented feed reaches 36.5 g / Kg; the feed quality is excellent.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a method and application for fermenting and degrading vomitoxin by various microorganisms. More specifically, it is the application of a strain of Bacillus velesi that degrades DON and its fermented feed to degrade microbial toxins. Background technique [0002] Deoxynivalenol (DON), also known as deoxynivalenol (DON), is a trichothecene toxin produced by the genus Fusarium. fungal diseases, leading to the contamination of the main grain grains with toxins after harvesting. At the same time, in the processing and storage of feed, food raw materials are prone to produce various mycotoxins due to mildew, among which DON contamination is the most widespread. DON is the most commonly polluted mycotoxin in feed and feed raw materials. It has been reported that in Shandong, Henan, Jiangsu, Northeast China and other regions in 2019, the detection rate of DON in feed and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00A23L5/20A23K10/12C12R1/07C12R1/25
CPCC12N1/20C12N15/01C12N13/00A23L5/28A23K10/12A23V2400/169Y02P60/87
Inventor 王德培赖根生刘子睦郭瑞薛鲜丽
Owner TIANJIN UNIV OF SCI & TECH
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