Establishment method of allogenic hematopoietic stem cell transplantation mouse experimental model

A technology of hematopoietic stem cells and a method for establishing them is applied in the field of establishing an experimental model of allogeneic hematopoietic stem cell transplantation in mice, which can solve the problems of large differences in research and other issues.

Pending Publication Date: 2022-07-08
农业农村部食物与营养发展研究所
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Problems solved by technology

The number of transplanted hematopoietic stem cells also determines the success rate of transplantation. The number of transplanted hematopoietic stem cells is relatively clear in clinical practice, but there are great differences in different studies on mice

Method used

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  • Establishment method of allogenic hematopoietic stem cell transplantation mouse experimental model
  • Establishment method of allogenic hematopoietic stem cell transplantation mouse experimental model
  • Establishment method of allogenic hematopoietic stem cell transplantation mouse experimental model

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Embodiment Construction

[0021] The principles and features of the present invention are described below, and the examples are only used to explain the present invention, but not to limit the scope of the present invention.

[0022] like figure 1 As shown, the following steps are included in this embodiment:

[0023] 1. Experimental animals

[0024] The donor mice were 6-8-week-old SPF-grade male C57BL / 6j mice (H-2kb), weighing 21-23 g; recipients were 6-8-week-old SPF-grade male BALB / c (H-2kd) mice , Weight 21-23g.

[0025] 2. Experimental grouping

[0026] In order to determine the optimal irradiation dose and the number of cells infused, two irradiation doses of 7.5Gy and 8.0Gy and 5.0×10 6 and 2.0×10 7 infusion of hematopoietic stem cells. The specific groups are as follows: 6 rats in the normal group (con), 12 rats in the irradiation non-transplantation group (RNT) (6 rats at 7.5G and 6 rats at 8.0Gy each), and the irradiated transplantation group includes RLTL (7.5Gy+5.0×10 rats). 6 ), RL...

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Abstract

The invention discloses a method for establishing an experimental model of an allogenic hematopoietic stem cell transplanted mouse, which comprises the following steps of: selecting inbred line C57BL/6 and BALB/c mice as donors and receptors of allogenic hematopoietic stem cell transplantation respectively, setting an irradiation dose of 8.0 Gy and a transplanted cell quantity gradient of 5.0 * 10 < 6 > to carry out modeling conditions, accelerating the reconstruction rate of an immune system after bone marrow transplantation, and establishing the experimental model of the allogenic hematopoietic stem cell transplantation mouse. The immune reconstruction time is shortened and the transplantation success rate is comprehensively improved.

Description

technical field [0001] The invention belongs to the technical field of construction of animal experimental models, in particular to a method for establishing a mouse experimental model of allogeneic hematopoietic stem cell transplantation. Background technique [0002] Allogeneic hematopoietic stem cell transplantation is an effective treatment for hematological malignancies such as leukemia and lymphoma. After transplantation, patients will face long-term immunodeficiency and are susceptible to opportunistic infections and cause a higher risk of mortality. After transplantation, the rate of hematopoietic reconstruction and the number of NK cells is relatively fast, and the normal hematopoietic function is basically restored within a few weeks, but the complete reconstruction of the number and function of T and B lymphocytes takes several months to several years. Recovery of the immune system, especially lower numbers of lymphocyte subsets, is strongly associated with lower ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87A01K67/027
CPCC12N15/87A01K67/0271A01K2207/12A01K2227/105A01K2267/03
Inventor 韩娟武小亮郭燕枝
Owner 农业农村部食物与营养发展研究所
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