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Polyvinyl alcohol/citric acid nanofiber membrane as well as preparation method and application thereof

A nanofiber membrane, polyvinyl alcohol technology, applied in the chemical characteristics of fibers, rayon manufacturing, single-component synthetic polymer rayon, etc. The effect of uniform fiber diameter distribution

Pending Publication Date: 2022-07-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the detection limit of the existing immunosensor for the detection of parathion needs to be further reduced, and the detection range needs to be further improved

Method used

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  • Polyvinyl alcohol/citric acid nanofiber membrane as well as preparation method and application thereof
  • Polyvinyl alcohol/citric acid nanofiber membrane as well as preparation method and application thereof
  • Polyvinyl alcohol/citric acid nanofiber membrane as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Preparation of parathion electrochemical immunosensor

[0070] S1. Add 3.2g of polyvinyl alcohol (PVA) into 20mL of distilled water, heat and stir at 80°C for about 4h to completely dissolve, cool, add 0.48g of citric acid (CA), stir at room temperature and dissolve completely to obtain 16% PVA / 15%CA solution, slowly fill the 16%PVA / 15%CA solution into a 10mL syringe to avoid the generation of air bubbles, fix the syringe filled with 16%PVA / 15%CA solution on the bolus slot, electrostatically The syringe pump of the spinning machine is connected to the positive pole of the high-voltage power supply, and the receiving plate is connected to the negative pole. The 20-gauge stainless steel needle is used to inject the sample. The injection rate is controlled by the syringe pump. The 15% CA solution was stretched and sprayed onto the receiving plate to form a nanofiber membrane;

[0071] The process parameters are: spinning voltage: 18kV, injection rate of syringe...

Embodiment 2

[0080] Example 2 Preparation of Electrochemical Immunosensor

[0081] The difference from Example 1 is:

[0082] S1. The time for thermal crosslinking is 4h.

[0083] The methods, reagents and addition amounts of the remaining steps are the same as those in Example 1.

Embodiment 3

[0134] Example 3 Study on the sensitivity and accuracy of parathion electrochemical immunosensor detection of parathion

[0135] Preparation of enzyme-labeled antibodies:

[0136] The anti-parathion nanobody VHH9 gene was transformed into Escherichia coli E.coli BL21 (DE3), and the anti-parathion nanobody VHH9 was obtained after expression and purification;

[0137] Weigh horseradish peroxidase (HRP) 12mg, dissolve it in 4mL acetate buffer (0.2moL / L, PH5.6), add freshly prepared 2mL NaIO dropwise 4 The solution (0.1moL / L) was stirred at 4°C in the dark for 30min, then 2mL of ethylene glycol solution (0.16moL / L) was added dropwise, reacted at room temperature for 30min, 6mg VHH9 antibody was added dropwise, stirred at 4°C for 30min, Dialyze with carbonate buffer (0.05mol / L, pH 9.6) overnight, remove the dialysate, add 0.4mL 5mg / mL NaBH dropwise 4 The solution was stirred at 4°C in the dark for 2h, then an equal volume of saturated ammonium sulfate solution was added, reacted ...

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Abstract

The invention belongs to the technical field of nanofiber membranes, and particularly relates to a polyvinyl alcohol / citric acid nanofiber membrane as well as a preparation method and application thereof. The polyvinyl alcohol / citric acid nanofiber membrane is prepared from polyvinyl alcohol and citric acid through an electrostatic spinning method and a thermal cross-linking reaction, the fiber diameter distribution in the membrane is uniform, the stable fiber morphology can be kept, the hydrophobicity of the membrane can be greatly improved, and the purpose of detecting parathion in an aqueous solution by the membrane is achieved; the electrochemical response speed is accelerated, and an electrochemical response signal is amplified; meanwhile, after the membrane is prepared into the parathion electrochemical immunosensor, the detection range is wide, the specificity is good, the sensitivity is high, the stability and the repeatability are good, the minimum detection limit is greatly reduced, the operation is simple and convenient, the cost is low, and the membrane can be widely applied to quantitative detection of parathion in vegetables and fruits.

Description

technical field [0001] The invention belongs to the technical field of nanofiber membranes. More specifically, it relates to a polyvinyl alcohol / citric acid nanofiber membrane and its preparation method and application. Background technique [0002] Parathion pesticides are widely used in the agricultural planting process to control insect pests, but parathion pesticides are acutely toxic to humans and animals, which can inhibit cholinesterase activity or destroy the endocrine system, thereby causing neurological disorders, language Disorders and other poisoning symptoms. my country has banned or restricted the use of parathion pesticides on vegetables, fruit trees, tea and Chinese herbal medicines in 2002, but poisoning incidents caused by parathion pesticides still occur from time to time, so it is necessary to establish an effective and rapid parathion pesticide. Testing methods to ensure dietary safety. [0003] At present, the detection methods of parathion pesticide ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N33/531D01F6/50D01F1/10
CPCG01N27/26G01N33/531D01F6/50D01F1/10Y02A50/30
Inventor 温棚尹文佳王弘
Owner SOUTH CHINA AGRI UNIV
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