Preparation method of silk fibroin/sodium alginate composite nanofiber scaffold

A technology of composite nanofibers and sodium alginate is applied in the fields of preparation and processing and utilization of fibroin, which can solve the problems of insufficient internal structure and performance of the scaffold, and achieve the effects of good mechanical properties, simple preparation process and easy control of conditions.

Inactive Publication Date: 2014-10-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are reports on the preparation of silk fibroin / sodium alginate porous scaffolds by freezing method (Kwang-Gill Lee et al., Journal of Applied Polymer Science 2004), these scaffolds have deficiencies in internal structure and performance

Method used

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  • Preparation method of silk fibroin/sodium alginate composite nanofiber scaffold
  • Preparation method of silk fibroin/sodium alginate composite nanofiber scaffold
  • Preparation method of silk fibroin/sodium alginate composite nanofiber scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Take 5ml of 3% silk fibroin solution, add 10ml of 0.5% sodium alginate solution in a 60°C water bath, and stir evenly with a glass rod.

[0025] (2) Add 5ml of dioxane / water mixture to the mixed solution, and stir well with a glass rod.

[0026] (3) The mixed solution obtained in step (2) was quickly moved into a -80°C refrigerator for aging for 4 hours to cause phase separation of the solution. After taking it out, let it stand at room temperature (25°C) for 30 minutes to form a gel.

[0027] (4) Soak the gel in excess absolute ethanol at -20°C for 24 hours.

[0028] (5) Pour off absolute ethanol, replace with deionized water at 4°C for 48 hours, and change the water continuously during this period.

[0029] (6) Pour off the deionized water, put it into a freeze dryer at -60°C for 24 hours, and prepare the silk fibroin / sodium alginate composite nanofibrous scaffold material SF / SA ( figure 1 ), the nanofiber diameter is 20-300nm.

Embodiment 2

[0031] (1) Take 5ml of 5% silk fibroin solution, add 10ml of 0.5% sodium alginate solution in a 60°C water bath, and stir evenly with a glass rod.

[0032] (2) Add 5ml of dioxane / water mixture to the mixed solution, and stir well with a glass rod.

[0033] (3) The mixed solution obtained in step (2) was quickly moved into a -80°C refrigerator for aging for 4 hours to cause phase separation of the solution. After taking it out, let it stand at room temperature (25°C) for 30 minutes to form a gel.

[0034] (4) Soak the gel in excess absolute ethanol at -20°C for 24 hours.

[0035] (5) Pour off absolute ethanol, replace with deionized water at 4°C for 48 hours, and change the water continuously during this period.

[0036] (6) Pour off the deionized water, put it into a freeze dryer at -60°C for 24 hours, and prepare the silk fibroin / sodium alginate composite nanofibrous scaffold material SF / SA ( figure 2 ), the nanofiber diameter is 40-200nm.

Embodiment 3

[0038] (1) Take 5ml of 7% silk fibroin solution, add 10ml of 0.5% sodium alginate solution in a 60°C water bath, and stir evenly with a glass rod.

[0039] (2) Add 5ml of dioxane / water mixture to the mixed solution, and stir well with a glass rod.

[0040] (3) The mixed solution obtained in step (2) was quickly moved into a -80°C refrigerator for aging for 4 hours to cause phase separation of the solution. After taking it out, let it stand at room temperature (25°C) for 30 minutes to form a gel.

[0041] (4) Soak the gel in excess absolute ethanol at -20°C for 24 hours.

[0042] (5) Pour off absolute ethanol, replace with deionized water at 4°C for 48 hours, and change the water continuously during this period.

[0043] (6) Pour off the deionized water, put it into a freeze dryer at -60°C for 24 hours, and prepare the silk fibroin / sodium alginate composite nanofibrous scaffold material SF / SA ( image 3 ), the nanofiber diameter is 30-300nm.

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Abstract

The invention relates to the field of biological scaffold material preparing and fibroin processing and utilizing, and in particular relates to a fibroin / sodium alginate composite nanofiber scaffold preparation method. The method comprises basic steps of: dissolving degummed fibrous fibroin into a LiBr solution with the concentration of 9mol / L, and controlling the temperature to be 37 DEG C, the time to be 1h; filtering, dialyzing, air drying, and concentratinng after the dissolving so as to obtain fibroin solutions with different weight concentrations; dissolving 500mg of sodium alginate into 100ml of deionized water, and dissolving in a water bath of 60 DEG C for 1h; and magnetically mixing the mixture to dissolve for 1h, so as to prepare a sodium alginate solution of 0.5%(w / v) and the like. A thermally induced phase separation method is adopted to prepare a fibroin / sodium alginate composite nanofiber scaffold material; and the method is simple in preparation technology, free from requirements on other complicated equipment, and low in cost and can perform large-scale batch production, and the conditions can be easily controlled.

Description

technical field [0001] The invention relates to the fields of preparation of biological scaffold materials and processing and utilization of silk protein, in particular to a preparation method of silk fibroin / sodium alginate composite nanofiber scaffold materials for tissue engineering. Background technique [0002] Tissue engineering technology is a new method for treating tissue and organ defects, which consists of biological scaffolds, cells, growth factors and other elements. Among them, biological scaffolds play an important role in tissue engineering as the physical support for cell growth, proliferation, and new tissue formation. The internal morphology of bioscaffold materials currently studied mainly includes sheet, porous, nanofiber and so on. Compared with sheet or porous materials, nanofibrous scaffolds have a structure and function closer to the extracellular matrix, and are more conducive to promoting cell adsorption, transfer, proliferation, and differentiati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/02A61L27/26
Inventor 朱良均刘小甜杨明英张海萍
Owner ZHEJIANG UNIV
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