A kind of antibacterial poly(ε-caprolactone)/mimetic antimicrobial peptide electrospun fiber membrane and preparation method thereof

A technology of electrospun fibers and antimicrobial peptides, applied in the field of preparation of biopolymer materials, to achieve the effect of adjustable hydrophilicity and hydrophobicity, beneficial to sterilization and healing, and obvious antibacterial activity

Inactive Publication Date: 2019-09-10
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the preparation of antibacterial electrospun fiber membranes by electrospinning using simulated antimicrobial peptides and poly(ε-caprolactone) as raw materials.

Method used

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  • A kind of antibacterial poly(ε-caprolactone)/mimetic antimicrobial peptide electrospun fiber membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of antibacterial poly(ε-caprolactone) / mimetic antimicrobial peptide electrospun fiber membrane

[0025] Configuration of the electrospinning solution: mix poly(ε-caprolactone) with a number average molecular weight of 80,000 and a polyhedral oligosilsesquioxane-polypeptide with a number average molecular weight of 8750 of the simulated antibacterial peptide at a mass ratio of 12:1 , dissolved in the solvent trifluoroethanol, and stirred for 48 hours to obtain an electrospinning solution with a uniform mass concentration of 130 mg / mL;

[0026] Inject the uniformly mixed electrospinning solution into a 5mL syringe, set the electrospinning voltage to 12kV, the flow rate of the electrospinning solution to 0.4mL / h, the receiving distance to 15cm, and the air humidity to 21%. Start the device for electrospinning, and obtain the PCL / mimetic antimicrobial peptide electrospun fiber membrane on the receiving device. The prepared poly(ε-caprolactone) / mimetic antim...

Embodiment 2

[0030] (1) Preparation of antibacterial poly(ε-caprolactone) / mimetic antimicrobial peptide electrospun fiber membrane

[0031] Mix poly(ε-caprolactone) with a number average molecular weight of 80,000 and a simulated antimicrobial peptide isobutyl-polypeptide with a number average molecular weight of 8080 at a mass ratio of 12:1, dissolve in the solvent trifluoroethanol, and stir for 54 hours , to obtain an electrospinning solution with a uniform mass concentration of 130 mg / mL;

[0032] Inject the homogeneously mixed electrospinning solution into a 5mL syringe, set the electrospinning voltage to 13kV, the flow rate of the electrospinning solution to 0.4mL / h, the receiving distance to 15cm, and the air humidity to 25%. The device was started to perform electrospinning, and poly(ε-caprolactone) / mimetic antimicrobial peptide electrospun fiber membrane was obtained on the receiving device. The prepared poly(ε-caprolactone) / mimetic antimicrobial peptide electrospun fiber membrane...

Embodiment 3

[0036] (1) Preparation of antibacterial poly(ε-caprolactone) / mimetic antimicrobial peptide electrospun fiber membrane

[0037] Poly(ε-caprolactone) with a number average molecular weight of 80,000 was mixed with polyethylene glycol-polypeptide with a number average molecular weight of 5320 of the simulated antimicrobial peptide at a mass ratio of 12:1, dissolved in the solvent trifluoroethanol, Stir for 42 hours to obtain an electrospinning solution with a uniform mass concentration of 130 mg / mL;

[0038] Inject the homogeneously mixed electrospinning solution into a 5mL syringe, set the electrospinning voltage to 12kV, the flow rate of the electrospinning solution to 0.4mL / h, the receiving distance to 15cm, and the air humidity to 18%. The device was started to perform electrospinning, and poly(ε-caprolactone) / mimetic antimicrobial peptide electrospun fiber membrane was obtained on the receiving device. The prepared poly(ε-caprolactone) / mimetic antimicrobial peptide electros...

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Abstract

The invention relates to an antibacterial poly(epsilon-caprolactone) / simulated antibacterial peptide electrospun fibrous membrane and a preparation method thereof, and belongs to the field of biological high polymer materials. The method comprises the steps that firstly, poly(epsilon-caprolactone) and simulated antibacterial peptide are mixed on the basis of the mass ratio being 24:1-3:1 and dissolved into trifluoroethanol to be stirred for 36-48 h, and an evenly-mixed elecrospun solution with the mass concentration being 100-160 mg / mL is prepared; then, the antibacterial poly(epsilon-caprolactone) / simulated antibacterial peptide electrospun fibrous membrane is prepared through the solution electrostatic spinning method. The method has the advantages that the process is simple, operation is easy and the cost is low. The electrospun fibrous membrane is adjustable in hydrophilicity and hydrophobicity, has the good cytocompatibility, meanwhile has the certain amount of simulated antibacterial peptide on the surface, has the obvious antibacterial activity on gram negative bacteria and gram positive bacteria, and is applicable to the field of biomedicine.

Description

technical field [0001] The invention belongs to the field of preparation of biopolymer materials, and relates to an antibacterial poly(ε-caprolactone) / simulated antibacterial peptide electrospun fiber membrane and a preparation method thereof. Background technique [0002] In the medical process, wound healing, grafts and medical instruments have bacterial infection, which poses a huge threat to the recovery of patients. In addition, bacterial infections are becoming more prevalent in areas such as pharmaceuticals, healthcare products and hygiene equipment, textiles, food packaging and food storage, and household appliances. Therefore, the development of new antibacterial materials plays a very important role in dealing with such problems. [0003] Electrospinning is a method that can prepare nanometer to micrometer ultrafine fiber membranes. Electrospun fiber membranes have high specific surface area and porosity, and are easy to achieve functionalization for different pu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/728D01D5/00
Inventor 袁晓燕姚添甜刘波李珍光周培琼赵蕴慧任丽霞
Owner TIANJIN UNIV
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