Method and device for constructing optic nerve injury model

Abstract

The invention belongs to the technical field of biomedical experiments, and relates to a construction method of an animal model, in particular to a method and a device for constructing an optic nerve injury model. An optic nerve injury model is constructed by using a specific device, and the method comprises the following steps: sleeving optic nerves with a pressurizing ring of the device behind a ball; the sutures located in the threading holes are aligned and knotted, and the circular ring is closed; a piston of the air injection cylinder is pushed to inject air into the pressurizing ring, and after air injection is completed, the piston is fixed; and after pressurization is completed, the piston is retracted, the suture is released after air in the pressurization ring is sucked out, and the pressurization ring is taken to construct the optic nerve injury model. According to the model, the injury degrees of the retinal ganglion cells at different parts of the retina approach to be consistent, and the injury to the arteriovenous blood vessel wall of the retina is reduced. The method disclosed by the invention is simple and feasible in process, and is easy to popularize in related researches of optic nerve injury diseases according to verification.

Description

technical field [0001] The invention belongs to the technical field of biomedical experiments, relates to a method for constructing an animal model, and in particular relates to a method and device for constructing an optic nerve injury model. Background technique [0002] Clinical practice shows that traumatic optic neuropathy (TON) is an irreversible blinding eye disease, and there is no good treatment method at present. The exploration of treatment strategies is of great significance. At present, there are several models for simulating clinical optic nerve injury diseases, such as optic nerve transection injury, optic nerve line injury, optic nerve bulb injury and optic nerve clamp injury, among which the optic nerve clamp injury model is the most widely used; The modeling method of the optic nerve clamp model is usually to use a clamping device with a head width of about 1-1.5mm (including vascular clamp, aneurysm clamp, optic nerve clamp, etc.) to clamp the experimenta...

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Problems solved by technology

[0004] The purpose of the present invention is based on the research status of the prior art, in order to overcome the existing problems of the prior art, aiming at the shortcomings of the current optic nerve clamp injury model, such as uneven stress on the optic nerve, easy damage to the central retinal artery and vein, etc., to provide a new Method for constructing animal model, especially method and device for constructing optic nerve injury model

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