Endophytic chaetomium globosum MG2 and application of endophytic chaetomium globosum MG2 in preventing and treating apple tree canker
A technology for endophytic Chaetomium globosa and apple tree rot, which is applied in the fields of application, microbe-based methods, and microbial measurement/inspection, and can solve the problems of ecological environment pollution, pesticide residues, and the enhancement of drug resistance of apple tree rot bacteria, etc. problem, to achieve the effect of ecological environment safety and strong specificity
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[0104] Preparation of SEM samples: After 2 to 3 days of flat plate confrontation culture, use a scalpel to cut the edge of normally growing V.mali hyphae as treatment A, and cut the edge of V.mali hyphae after antagonism of Chaetomium globosa as treatment B, Placed in 4% (v / v) glutaraldehyde phosphate buffer solution at 4°C for 20 h; washed with pH 6.8 PBS buffer for 4 times, each time interval 20 min; samples were dehydrated by serial ethanol, replaced with isoamyl acetate, CO 2 After critical point drying, sticking, and gold plating, observation and photography were performed under a Hitachi S-4800 field emission scanning electron microscope; wherein, the concentration of the glutaraldehyde phosphate buffer solution was 100 mM, pH 6.8.
[0105] The pathogenicity detection of the isolated apple branches and leaves by Chaetomium globosa in step (2) provided in the embodiment of the present invention includes:
[0106] Branch treatment: Collect 1-2 year old Fuji apple branches...
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