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Endophytic chaetomium globosum MG2 and application of endophytic chaetomium globosum MG2 in preventing and treating apple tree canker

A technology for endophytic Chaetomium globosa and apple tree rot, which is applied in the fields of application, microbe-based methods, and microbial measurement/inspection, and can solve the problems of ecological environment pollution, pesticide residues, and the enhancement of drug resistance of apple tree rot bacteria, etc. problem, to achieve the effect of ecological environment safety and strong specificity

Pending Publication Date: 2022-07-22
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] (1) Due to the long-term and extensive use of chemical fungicides, especially systemic fungicides, the drug resistance of apple tree rot bacteria has increased, ecological environment pollution and pesticide residues have seriously restricted the healthy development of green food
[0005] (2) At present, most of the biological control fungal resources are still in the initial stage of research and development, especially the lack of specific and effective biocontrol products, which are far from meeting the actual requirements of apple tree rot disease control in production.

Method used

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  • Endophytic chaetomium globosum MG2 and application of endophytic chaetomium globosum MG2 in preventing and treating apple tree canker
  • Endophytic chaetomium globosum MG2 and application of endophytic chaetomium globosum MG2 in preventing and treating apple tree canker
  • Endophytic chaetomium globosum MG2 and application of endophytic chaetomium globosum MG2 in preventing and treating apple tree canker

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preparation example Construction

[0104] Preparation of SEM samples: After 2 to 3 days of flat plate confrontation culture, use a scalpel to cut the edge of normally growing V.mali hyphae as treatment A, and cut the edge of V.mali hyphae after antagonism of Chaetomium globosa as treatment B, Placed in 4% (v / v) glutaraldehyde phosphate buffer solution at 4°C for 20 h; washed with pH 6.8 PBS buffer for 4 times, each time interval 20 min; samples were dehydrated by serial ethanol, replaced with isoamyl acetate, CO 2 After critical point drying, sticking, and gold plating, observation and photography were performed under a Hitachi S-4800 field emission scanning electron microscope; wherein, the concentration of the glutaraldehyde phosphate buffer solution was 100 mM, pH 6.8.

[0105] The pathogenicity detection of the isolated apple branches and leaves by Chaetomium globosa in step (2) provided in the embodiment of the present invention includes:

[0106] Branch treatment: Collect 1-2 year old Fuji apple branches...

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Abstract

The invention belongs to the technical field of biological prevention and control of plant diseases, and discloses endophytic chaetomium globosum MG2 and application thereof in prevention and control of apple tree canker, the endophytic chaetomium globosum MG2 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40070; the endogenous chaetomium globosum MG2 is separated from healthy chaenomeles speciosa barks, is identified and determined as chaetomium globosum, and is stored on a small inclined plane at 4 DEG C. Experimental results show that the endogenous chaetomium globosum inhibits spore germination of valsa ceratosperma, and fermentation liquor has good thermal stability; the chaetomium globosum fermentation liquor has good thermal stability and ultraviolet stability and can be applied to fields; the chaetomium globosum and the fermentation liquor thereof have certain protection and treatment effects on the apple in-vitro branches, but the protection effect is better than the treatment effect, and a foundation is laid for later production of chaetomium globosum prevention and protection preparations.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control, and in particular relates to a strain of Chaetomium endophytes MG2 and its application in preventing and treating apple tree rot disease. Background technique [0002] my country is the world's largest apple producer, and the apple industry is an important pillar industry for rural revitalization. However, apple tree rot, caused by the fungus Valsa mali, has been threatening the health of the industry. Rot disease is the most devastating branch disease of apples. It often causes dead branches and trees, and even destroys orchards in severe cases, resulting in huge economic losses. At present, the prevention and control of apple tree rot disease mainly relies on chemical agents. Due to the long-term and extensive use of chemical fungicides, especially systemic fungicides, the problem of drug resistance of pathogens has become increasingly prominent. There is an urgent ne...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q1/04C12Q1/6895C12Q1/686C12Q1/6869A01N63/30A01P3/00C12R1/645
CPCC12Q1/04C12Q1/6895C12Q1/686C12Q1/6869A01N63/30G01N2333/37Y02A50/30
Inventor 冯浩渠非黄丽丽贺艳婷苏晓州
Owner NORTHWEST A & F UNIV
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