Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for silencing long non-coding RNA expression and application thereof

A long-chain non-coding and non-coding technology, applied in the biological field, can solve the problems of affecting expression and difficult operation.

Pending Publication Date: 2022-07-29
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods have major defects. First, the operation is difficult. Second, there are many transcriptional regulatory elements in the promoter region. Knocking out this region may affect the expression of adjacent genes, especially NAT.
Secondly, there are many introns in eukaryotic genes, and the knockout of the whole gene may affect the expression of genes near the gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for silencing long non-coding RNA expression and application thereof
  • Method for silencing long non-coding RNA expression and application thereof
  • Method for silencing long non-coding RNA expression and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1. Novel strategies for silencing long non-coding RNAs and protein-coding genes in eukaryotic genomes

[0089]The present invention provides a special method for silencing the expression of long non-coding RNAs, called precursor RNA splicing interference method (IE method). This method targets the region of lncRNA BPS to 3'SS (based on CRISPR / Cas9 technology, designed paired sgRNA) is cleaved and repaired by a non-homologous end joining (NHEJ) mechanism in the host cell, resulting in two cleavage sites Deletion of the inter-BPS-3'SS segment or insertion of other sequences, thereby interfering with the splicing of precursor RNAs of related genes and causing the degradation of splicing-impaired precursor RNAs, while reducing or avoiding contralateral or adjacent genes. interference. At the same time, this method is also applicable to the silencing of protein-coding genes.

[0090] Tested eukaryotic cells: HPDE6-C7 cells, 293Cells, low passage cells and Hela cell...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for silencing long non-coding RNA expression and application thereof. The invention provides a pair of sgRNAs for silencing long non-coding RNA expression in a eukaryotic cell genome, one of the sgRNAs targets a sequence upstream of a BPS site of any intron of the long non-coding RNA, and the other one of the sgRNAs targets a sequence downstream of a 3 'SS site of the same intron of the long non-coding RNA. According to the invention, the technical difficulty and interference to nearby genes caused by the fact that excision of a promoter region or insertion of a polyA sequence or excision of a full-length gene has to be adopted in the prior art to silence long non-coding RNA expression are overcome.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for silencing long non-coding RNA expression and its application. Background technique [0002] Long non-coding RNAs (lncRNAs) are a class of RNAs with a length of more than 200 nt, most of which are not translated due to the lack of open reading frames. Before this kind of RNA was poorly understood, it was considered to be the "dark matter" in the cell, but with the study of RNA function, more and more evidences show that this kind of RNA also plays a special biological function. Regulation of gene expression is a reusable technical approach to study gene function. Compared with siRNA and ASO, CRISPR method is a more mature and effective technical method for regulating genes, especially lncRNAs located in the nucleus. However, due to the complexity of the location between genes, the application of CRISPR method is greatly limited. Most of these lncRNAs that cannot be edi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/85C12N15/55C12N15/87C12N9/22
CPCC12N15/113C12N15/85C12N15/87C12N9/22C12N2310/20C12N2310/11C12N2800/107
Inventor 张雅鸥张世宽王松茂
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com