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Amorphophallus rivieri group seedling-culturing batch production and cultivation technology

A technology of tissue culture seedlings and mass production, which is applied in the field of agricultural biology and agricultural cultivation, can solve the problems of mass production of konjac tissue culture seedlings, etc., and achieve the effect of low cost and short growth cycle

Inactive Publication Date: 2004-06-23
云南省农业科学院生物技术与种质资源研究所
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AI Technical Summary

Problems solved by technology

So far, there have been no public reports that mass production of konjac tissue culture seedlings can be achieved, and that the average weight of the harvested tubers in the year is more than 50g, and one seedling can produce a large tuber and 2 to 4 small taro

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0007] First wash the tuber-shaped konjac explants, and take the terminal bud part according to the specification of 0.5cm×0.5cm, and then soak them in 75% alcohol for 2-3 seconds and 0.1% mercury liter for 15 minutes, and then use aseptic Rinse with water, put the konjac tuber explants into MS+6BA after washing 6～0.1 mg / L+NAA 6～0 mg / L, PH5.8 medium, cultured in an environment with a temperature above 20°C and a light of 1000LX for 45 days, the seedlings can be grown in one step. At this time, the tissue-cultured seedlings were put into the same medium for subculture, once every 20 days, and the multiplication ratio was 1:4-1:6. During the subculture process, the temperature is above 20°C and the light is 1000LX. When the seedlings grow to a height of 3-4cm, cut the branches and put them into MS+NAA 5～1 mg / L rooting culture medium for 12 to 15 days, after the root grows out of the bottle, the control of temperature and light is the same as the subculture process. Before tr...

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Abstract

The present invention adopts identical culture medium to make inoculation and subculture according to the conventional operation condition, it can obtain seedling by one step in only 45 days, then one subculture is achieved at an interval of 20 days, its reproduction multiples are 1:4 or 1:6; then the rooting culture brings about rooting in 12-15 days, and the obtained young plant with culture stem of cleaned root portion is stably placed in the seminary, and under the conditions of that the temp. is 20-30 deg.C. humidity is 80-90% and sunshading degree is 50%, the seedling grows up, and at the same time, 1-6 ppm of paclobutrazol and nutrient solution are applied. Said invention can implement batch production of group-cultured seedlings, its growing period is short, cost is low, and it can obtain above 50% of seed konjak in current year.

Description

Technical field: [0001] The invention relates to a batch production of konjac tissue culture seedlings and a technique for cultivating tissue culture seedlings, belonging to agricultural biology and agricultural cultivation [0002] technology field. Background technique: [0003] Because the glucomannan contained in konjac has extensive and special uses, it has attracted the interest and attention of many countries. Since the end of the 1980s, people have been using the two-step seedling method to do konjac tissue culture work, and have done research on various factors that affect the proliferation and rooting of konjac tissue culture, and moved a small amount of tissue culture seedlings into field cultivation. And obtained about 8~30g taro seed, and the tuber (kind of taro) that is used for production needs the growth period of three years. The above tests are limited to laboratory research, and the cost of the test is relatively high. So far, there have been no public ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王玲李勇军陈丽华房亚南
Owner 云南省农业科学院生物技术与种质资源研究所
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