Method for quantitative analyzing L-and D-type lactata in rumen liquid or greenfeed fermented liquid
A technology for silage and quantitative analysis, applied in the field of lactic acid analysis, can solve problems such as inability to determine the ratio of enantiomers, and achieve the effects of low cost, safe use and simple operation
Inactive Publication Date: 2005-12-21
CHINA AGRI UNIV
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Problems solved by technology
However, the problems in these technical solutions are: (1) the chemical method can only measure the total amount of lactic acid, and cannot determine the ratio of the two enantiomers
However, the problem of using the enantiomeric characteristics of L-menthol itself to carry out esterification with lactic acid to realize the complete resolution and quantitative detection of L- and D-lactic acid on gas chromatography has not attracted people's attention
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[0017] The present invention provides a method for quantitative analysis of L- and D-type lactic acid in rumen juice or silage fermentation broth with low cost and high sensitivity through the esterification reaction of a common chiral reagent with lactic acid. Its characteristics are It is: in the lactic acid extracted from the rumen juice or silage fermentation broth, the chiral reagent L-menthol is added as a derivatization reagent, and the corresponding isomer of lactic acid undergoes an esterification reaction to obtain menthyl lactate, and then in the gas phase Analyze on the chromatograph, showing the different spectra corresponding to the corresponding isomers of lactic acid L- and D- lactic acid. According to this spectrogram, the L- and D- lactic acid in the rumen fluid or silage fermentation broth can be resolved and quantitatively analyzed. , The analysis process is as follows:
[0018] 1). Extracting lactic acid from rumen juice or silage fermentation broth: Centrifug...
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The invention discloses a quantitative analysis method for L- and D-type lactic acid in rumen fluid or silage fermented liquid, which belongs to the technical scope of lactic acid analysis. The method utilizes the chiral reagent L-menthol as a derivatization reagent to carry out esterification reaction with lactic acid enantiomers to obtain menthyl lactate, which is then analyzed on a gas chromatograph, showing that the corresponding lactic acid enantiomers L- and Different spectrograms of D-type lactic acid, according to which spectrograms are split and quantitatively analyzed to obtain L- and D-type lactic acid in rumen juice or silage fermentation broth and calculate the content of L-lactic acid and D-lactic acid. The problems of complex operation, high cost, low sensitivity and incomplete separation of L- and D-lactic acid in the prior art solutions are solved. The method has the advantages of low cost, complete resolution, high sensitivity and simultaneous determination of lactic acid enantiomers and other short-chain fatty acids. The technical scheme is mainly used for the determination of rumen fermentation parameters of cattle and sheep and other ruminants and the quality evaluation of silage.
Description
Technical field [0001] The invention belongs to the technical scope of lactic acid analysis, and relates to a quantitative analysis method of L- and D-type lactic acid in rumen juice or silage fermentation broth. Background technique [0002] Ruminants such as cattle and sheep rely on the fermentation of rumen microorganisms to obtain the nutrients they need. As people's demand for high-quality beef, mutton and dairy products increases, a large amount of starchy carbohydrate feed and high-quality silage are bound to be used in the feeding process of ruminants such as cattle and sheep. [0003] Starchy carbohydrate feed is decomposed to produce volatile acid, lactic acid and other short-chain fatty acids under the fermentation of rumen microorganisms, which is the energy source of animals. Under normal fermentation conditions, the lactic acid in the rumen is mainly L-lactic acid, and D-lactic acid accounts for a small proportion (about 20% of the total lactic acid, or even none); ...
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IPC IPC(8): G01N30/00
Inventor 常影邱静孟庆翔周志强
Owner CHINA AGRI UNIV